Enhanced leavening properties of baker’s yeast overexpressing MAL62 with deletion of MIG1 in lean dough
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This study aimed to increase maltose fermentation in industrial baker’s yeast to increase its leavening properties. To this end, we overexpressed MAL62 encoding alpha-glucosidase (maltase) and deleted MIG1 encoding a transcriptional repressor that regulates MAL gene expression. Strain overexpressing MAL62 showed 46.3 % higher alpha-glucosidase activity and enhanced leaving activity than the parental strain when tested in glucose–maltose low sugar model liquid dough (LSMLD). Deleting MIG1 was much less effective, but it could further strengthen leavening properties in a strain overexpressing MAL62. The relationship between maltose permease and alpha-glucosidase was further dissected by transforming the two genes. The results indicated that without increasing the maltose permease activity, maltose metabolism could also be enhanced by the increased alpha-glucosidase activity. Previous strategies for strain improvement have targeted the enhancement of alpha-glucosidase and maltose permease activities in concert. Our results suggest that increasing alpha-glucosidase activity is sufficient to improve maltose fermentation in lean dough.
KeywordsAlpha-glucosidase activity Baker’s yeast Maltose metabolism MIG1 Fermentation
The current study was financially supported by the National Natural Science Foundation of China (31000043), Major Project of Research Program on Applied Fundamentals and Advanced Technologies of Tianjin (10JCZDJC16700), and program for Changjiang Scholars and Innovative Research Team in University (IRT1166).
- 9.Higgins VJ, Braidwood M, Bell P, Bissinger P, Dawes IW, Attfield PV (1999) Genetic evidence that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker’s yeast in unsugared dough. Appl Environ Microbiol 65:680–685PubMedGoogle Scholar
- 17.Lilly M, Lambrechts MG, Pretorius IS (2000) Effect of increased yeast alcohol acetyltransferase activity on flavor profiles of wine and distillates. Appl Environ Microbiol 66:744–753Google Scholar