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Journal of Industrial Microbiology & Biotechnology

, Volume 38, Issue 10, pp 1657–1665 | Cite as

Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli

  • Oksik Choi
  • Cheng-Zhu Wu
  • Sun Young Kang
  • Jong Seog Ahn
  • Tai-Boong Uhm
  • Young-Soo HongEmail author
Original Paper

Abstract

Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artificial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established for production of plant-specific metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artificial pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor, caffeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea.

Keywords

Biosynthesis Phenylpropanoid Heterologous expression Artificial pathway 

Notes

Acknowledgments

This work was supported in part by the 21C Frontier Microbial Genomics and Application Center, Basic Science Research Program, and Global R&D Center program, the Ministry of Science and Technology, Republic of Korea, and by a grant from KRIBB Research Initiative Program. The authors would also like to thank Joon-Tae Park for assistance in the LC–MS experiment.

Supplementary material

10295_2011_954_MOESM1_ESM.doc (27 kb)
Supplementary material 1 (doc 27 kb)
10295_2011_954_MOESM2_ESM.pptx (604 kb)
Supplementary material 2 (pptx 603 kb)

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Copyright information

© Society for Industrial Microbiology 2011

Authors and Affiliations

  • Oksik Choi
    • 1
    • 2
  • Cheng-Zhu Wu
    • 1
  • Sun Young Kang
    • 1
  • Jong Seog Ahn
    • 1
  • Tai-Boong Uhm
    • 2
  • Young-Soo Hong
    • 1
    Email author
  1. 1.Korea Research Institute of Bioscience and BiotechnologyChungbukRepublic of Korea
  2. 2.Biological SciencesChunbuk National UniversityJeollabuk-doRepublic of Korea

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