Effect of calcifying bacteria on permeation properties of concrete structures
Microbially enhanced calcite precipitation on concrete or mortar has become an important area of research regarding construction materials. This study examined the effect of calcite precipitation induced by Sporosarcina pasteurii (Bp M-3) on parameters affecting the durability of concrete or mortar. An inexpensive industrial waste, corn steep liquor (CSL), from starch industry was used as nutrient source for the growth of bacteria and calcite production, and the results obtained with CSL were compared with those of the standard commercial medium. Bacterial deposition of a layer of calcite on the surface of the specimens resulted in substantial decrease of water uptake, permeability, and chloride penetration compared with control specimens without bacteria. The results obtained with CSL medium were comparable to those obtained with standard medium, indicating the economization of the biocalcification process. The results suggest that calcifying bacteria play an important role in enhancing the durability of concrete structures.
KeywordsCalcite precipitation Sporosarcina pasteurii Industrial waste Corn steep liquor Permeability Chloride penetration
Financial assistance for this study received from the Atomic Energy Regulatory Board, Department of Atomic Energy and Department of Science & Technology, India, is gratefully acknowledged. The authors thank TIFAC-CORE for the experimental facilities.
- 4.American Public Health Association (APHA) (1989) Standard methods for the examination of water and wastewater, 17th edn. American Public Health Association, WashingtonGoogle Scholar
- 16.Kristiansen B (2001) Process economics. In: Ratledge C, Kristiansen B (eds) Biotechnology, 2nd edn. Cambridge University Press, CambridgeGoogle Scholar
- 20.Ramachandran SK, Ramakrishnan V, Bang SS (2001) Remediation of concrete using microorganisms. ACI Mater J 98:3–9Google Scholar
- 23.Tiano P, Biagiotti L, Mastromei G (1999) Bacterial bio-mediated calcite precipitation for monumental stones conservation: methods of evaluation. J Microbiol Method 36:138–145Google Scholar