Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena

  • Oscar A. Rojas-Rejón
  • Héctor M. Poggi-Varaldo
  • Ana C. Ramos-Valdivia
  • Alfredo Martínez-Jiménez
  • Eliseo Cristiani-Urbina
  • Mayra de la Torre Martínez
  • Teresa Ponce-Noyola
Original Paper

Abstract

Derepressed mutant PR-22 was obtained by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) mutagenic treatment of Cellulomonas flavigena PN-120. This mutant improved its xylanolytic activity from 26.9 to 40 U mg−1 and cellulolytic activity from 1.9 to 4 U mg−1; this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. Either glucose or cellobiose was added to cultures of C. flavigena PN-120 and mutant PR-22 induced with sugarcane bagasse in batch culture. The inhibitory effect of glucose on xylanase activity was more noticeable for parent strain PN-120 than for mutant PR-22. When 20 mM glucose was added, the xylanolytic activity decreased 41% compared to the culture grown without glucose in mutant PR-22, whereas in the PN-120 strain the xylanolytic activity decreased by 49% at the same conditions compared to its own control. Addition of 10 and 15 mM of glucose did not adversely affect CMCase activity in PR-22, but glucose at 20 mM inhibited the enzymatic activity by 28%. The CMCase activity of the PN-120 strain was more sensitive to glucose than PR-22, with a reduction of CMCase activity in the range of 20–32%. Cellobiose had a more significant effect on xylanase and CMCase activities than glucose did in the mutant PR-22 and parent strain. Nevertheless, the activities under both conditions were always higher in the mutant PR-22 than in the PN-120 strain. Enzymatic saccharification experiments showed that it is possible to accumulate up to 10 g l−1 of total soluble sugars from pretreated sugarcane bagasse with the concentrated enzymatic crude extract from mutant PR-22.

Keywords

Cellulomonas flavigena Mutant Derepressed Saccharification Sugarcane bagasse Cellulase Xylanase 

Notes

Acknowledgments

This work was supported by Consejo Nacional de Ciencia y Tecnología México (CONACYT) (Grant 104333).

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Copyright information

© Society for Industrial Microbiology 2010

Authors and Affiliations

  • Oscar A. Rojas-Rejón
    • 1
  • Héctor M. Poggi-Varaldo
    • 1
  • Ana C. Ramos-Valdivia
    • 1
  • Alfredo Martínez-Jiménez
    • 2
  • Eliseo Cristiani-Urbina
    • 3
  • Mayra de la Torre Martínez
    • 4
  • Teresa Ponce-Noyola
    • 1
  1. 1.Departamento de Biotecnología y BioingenieríaCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalZacatencoMexico
  2. 2.Instituto de Biotecnología UNAMCuernavacaMexico
  3. 3.Escuela Nacional de Ciencias Biológicas del IPN, Prolongación Carpio y Plan de AyalaPlutarco Elías CallesMexico
  4. 4.Centro de Investigación en Alimentación y DesarrolloHermosilloMexico

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