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Journal of Industrial Microbiology & Biotechnology

, Volume 37, Issue 11, pp 1111–1119 | Cite as

The role of surface charge and hydrophobicity in the attachment of Anoxybacillus flavithermus isolated from milk powder

  • J. S. Palmer
  • S. H. Flint
  • J. Schmid
  • J. D. Brooks
Original Paper

Abstract

The aim of the present study was to investigate the attachment mechanisms that enable the thermophile Anoxybacillus flavithermus (B12) to attach to stainless-steel surfaces. Passing a B12 culture through a column of stainless-steel chips, collecting the first cells to pass through, re-culturing, and repeating the process six times, resulted in the isolation of a mutant, labeled X7, with tenfold reduced ability to attach to stainless steel as well as a reduced ability to attach to plastic. A comparison of bacterial cell-surface properties indicated that X7 was less hydrophobic than its parental strain B12. Cell-surface charge measurements also suggest that X7 had a lower net-negative surface charge. Disruption of extracellular polysaccharides and DNA appeared to have no effect on the attachment process. Removal of surface proteins caused a reduction in attachment of both B12 and X7, suggesting surface protein involvement in attachment.

Keywords

Anoxybacillus flavithermus Biofilm Dairy Surface charge Hydrophobicity 

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Copyright information

© Society for Industrial Microbiology 2010

Authors and Affiliations

  • J. S. Palmer
    • 1
  • S. H. Flint
    • 1
  • J. Schmid
    • 2
  • J. D. Brooks
    • 3
  1. 1.Institute of Food, Nutrition and Human HealthMassey UniversityPalmerston NorthNew Zealand
  2. 2.Institute of Molecular BiosciencesMassey UniversityPalmerston NorthNew Zealand
  3. 3.School of Applied SciencesAuckland University of TechnologyAucklandNew Zealand

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