Microbial production of 2,3-butanediol by a surfactant (serrawettin)-deficient mutant of Serratia marcescens H30

  • Liaoyuan Zhang
  • Jian’an Sun
  • Yingli Hao
  • Jiawen Zhu
  • Ju Chu
  • Dongzhi Wei
  • Yaling Shen
Original Paper


Serrawettin W1 produced by Serratia marcescens is a surface-active exolipid resulting in a lot foam formation during the 2,3-butanediol (2,3-BD) fermentation process. In order to avoid excessive addition of antifoam agent and microbial contamination, S. marcescens mutants deficient in serrawettin W1 formation were successfully constructed through insertional inactivation of the swrW gene coding for serrawettin W1 synthase. The shake flask and batch experiments suggested that disruption of the swrW gene led to significant reduction of the foam formation and improved 2,3-BD production a little. Ultimately, fed-batch culturing of the mutant afforded a maximum 2,3-BD concentration of 152 g l−1 with a productivity of 2.67 g l−1 h−1 and a yield of 92.6% at 57 h.


2,3-Butanediol Serrawettin W1 synthase Inactivation Serratia marcescens Fed-batch 



This work was supported by the Chinese National Program for High Technology and Development (863 Program) (No. 2006AA02Z243), Shanghai Leading Academic Discipline Project (Project B505), and State Key Laboratory special fund (No. 2060204).


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Copyright information

© Society for Industrial Microbiology 2010

Authors and Affiliations

  1. 1.State Key Laboratory of Bioreactor Engineering, New World Institute of BiotechnologyEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  2. 2.Chemical Engineering Research CenterEast China University of Science and TechnologyShanghaiPeople’s Republic of China

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