Abstract
Moenomycin, a natural phosphoglycolipid product that has a long history of use in animal nutrition, is currently considered an attractive starting point for the development of novel antibiotics. We recently reconstituted the biosynthesis of this natural product in a heterologous host, Streptomyces lividans TK24, but production levels were too low to be useful. We have examined several other streptomycetes strains as hosts and have also explored the overexpression of two pleiotropic regulatory genes, afsS and relA, on moenomycin production. A moenomycin-resistant derivative of S. albus J1074 was found to give the highest titers of moenomycin, and production was improved by overexpressing relA. Partial duplication of the moe cluster 1 in S. ghanaensis also increased average moenomycin production. The results reported here suggest that rational manipulation of global regulators combined with increased moe gene dosage could be a useful technique for improvement of moenomycin biosynthesis.
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This work was supported by grant Bg-01F from the Ministry of Education and Science of Ukraine (to V. F.) and NIH grant AI50855 (to S. W.).
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Makitrynskyy, R., Rebets, Y., Ostash, B. et al. Genetic factors that influence moenomycin production in streptomycetes. J Ind Microbiol Biotechnol 37, 559–566 (2010). https://doi.org/10.1007/s10295-010-0701-1
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DOI: https://doi.org/10.1007/s10295-010-0701-1