Antimicrobial compounds produced by Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian meat product

  • Kátia G. de Carvalho
  • Felipe H. S. Bambirra
  • Monika F. Kruger
  • Matheus S. Barbosa
  • Jamil S. Oliveira
  • Alexandre M. C. Santos
  • Jacques R. Nicoli
  • Marcelo P. Bemquerer
  • Antonio de Miranda
  • Emiliano J. Salvucci
  • Fernando J. M. Sesma
  • Bernadette D. G. M. Franco
Original Paper

Abstract

Bacteriocins produced by lactic acid bacteria are gaining increased importance due to their activity against undesirable microorganisms in foods. In this study, a concentrated acid extract of a culture of Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian pork product, was purified by cation exchange and reversed-phase chromatographic methods. The amino acid sequences of the active antimicrobial compounds determined by Edman degradation were compared to known protein sequences using the BLAST-P software. Three different antimicrobial compounds were obtained, P1, P2 and P3, and mass spectrometry indicated molecular masses of 4.4, 6.8 and 9.5 kDa, respectively. P1 corresponds to classical sakacin P, P2 is identical to the 30S ribosomal protein S21 of L. sakei subsp. sakei 23 K, and P3 is identical to a histone-like DNA-binding protein HV produced by L. sakei subsp. sakei 23 K. Total genomic DNA was extracted and used as target DNA for PCR amplification of the genes sak, lis and his involved in the synthesis of P1, P2 and P3. The fragments were cloned in pET28b expression vector and the resulting plasmids transformed in E. coli KRX competent cells. The transformants were active against Listeria monocytogenes, indicating that the activity of the classical sakacin P produced by L. sakei 2a can be complemented by other antimicrobial proteins.

Keywords

Lactobacillus sakei 2a Bacteriocins Antimicrobial proteins Lactic acid bacteria 

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Copyright information

© Society for Industrial Microbiology 2009

Authors and Affiliations

  • Kátia G. de Carvalho
    • 1
    • 6
  • Felipe H. S. Bambirra
    • 3
  • Monika F. Kruger
    • 1
  • Matheus S. Barbosa
    • 1
  • Jamil S. Oliveira
    • 2
  • Alexandre M. C. Santos
    • 2
    • 7
  • Jacques R. Nicoli
    • 3
  • Marcelo P. Bemquerer
    • 2
    • 4
  • Antonio de Miranda
    • 5
  • Emiliano J. Salvucci
    • 6
  • Fernando J. M. Sesma
    • 6
  • Bernadette D. G. M. Franco
    • 1
  1. 1.Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências FarmacêuticasUniversidade de São PauloSão PauloBrazil
  2. 2.Departamento de Bioquímica e Imunologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  3. 3.Departamento de Microbiologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  4. 4.Embrapa Recursos Genéticos e Biotecnologia Parque Estação BiológicaBrasíliaBrazil
  5. 5.Departamento de BiofisicaUniversidade Federal de São PauloSão PauloBrazil
  6. 6.Centro de Referencia para Lactobacilos (CERELA)TucumánArgentina
  7. 7.Departamento de Ciências FisiológicasUniversidade Federal do Espírito SantoVitóriaBrazil

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