Decolorization of Direct Red 28 by mixed bacterial culture in an up-flow immobilized bioreactor

Original Paper


Aerobic mixed bacterial culture comprised of five isolates (Bacillus vallismortis, B. pumilus, B. cereus, B. subtilis and B. megaterium) identified by 16srDNA analysis was developed from wastewater samples from the aeration tank of an effluent treatment plant of a textile and dyeing industry and evaluated for its ability to decolorize azo dye Direct Red 28 in an up-flow immobilized packed bed bioreactor using marble chips as support matrix. The bioreactor was operated under two parameters: an aeration rate of 0.4 and 0.6 mmol/min at a flow rate of 60, 90 and 120 ml/h, respectively. At a constant aeration rate of 0.4 mmol/min and with flow rates of 60, 90 and 120 ml/h, optimum decolorization of 91, 75 and 72% was observed, while at an aeration rate of 0.6 mmol/min and flow rates of 60, 90 and 120 ml/h, optimum decolorization of 93, 78 and 72% was observed over 10 days. The study concluded that across the two aeration rates and the respective flow rates, the higher aeration rate of 0.6 mmol/min along with a flow rate of 60 ml/h was best suited to decolorize Direct Red 28 in the packed bed bioreactor. Spectral changes of the input and output of the bioreactor by UV–visible spectroscopy indicated decolorization of the dye solution by degradation in addition to the visual observation of the biosorption process.


Direct Red 28 Biofilm Mixed bacterial culture Up-flow immobilized bioreactor Bacillus sp. 



We express our gratitude to the Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, for financial support for this research. The authors are also thankful for the necessary equipment and facilities provided by the Department of Biotechnology and Environmental Sciences and TIFAC-CORE, Thapar University, Punjab, where this work was carried out.


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Copyright information

© Society for Industrial Microbiology 2009

Authors and Affiliations

  • Bella Devassy Tony
    • 2
  • Dinesh Goyal
    • 2
  • Sunil Khanna
    • 1
  1. 1.Department of Biotechnology and BioinformaticsNIIT Institute of Information TechnologyNew DelhiIndia
  2. 2.Department of Biotechnology and Environmental SciencesThapar UniversityPatialaIndia

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