Influence of metal addition on ethanol production with Pichia stipitis ATCC 58784

  • Yuan Li
  • Zhenya Zhang
  • Zhongfang Lei
  • Yingnan Yang
  • Motoo Utsumi
  • Norio Sugiura
Original Paper

Abstract

Trace metals always act as cofactors or coenzymes in many cellular processes. Deficiency or excess of some metals will affect the fermentation of lignocellulosic hydrolysate. In order to make sure the deficient or excessive states of metals in culture medium, metal contents analysis was conducted in Pichia stipitis ATCC 58784 cells, synthetic medium, and diluted acid hydrolysate of rice straw. The results showed that Cu, Ni, and Co were deficient, and Al was a little excessive. So the influences of Cu2+, Al3+, Ni2+, and Co2+ additions on the growth and ethanol production of ATCC 58784 were further researched. Low concentration additions of Cu2+ and Al3+ (<0.24 mM and <0.23 mM, respectively) improved biomass growth of ATCC 58784 by 34 and 13%, respectively; however, higher concentrations decreased biomass growth. On the other hand, addition of Cu2+ (0.39 mM) did not affect volumetric ethanol production significantly (P = 0.05) and addition of Al3+ (0.38 mM) showed no influence on volumetric ethanol production (P = 0.68). Addition of 0.074 mM Co2+ inhibited biomass growth of ATCC 58784 by 13% and volumetric ethanol production by 10%. The biomass growth and volumetric ethanol production of ATCC 58784 was arrested by the addition of 0.33 mM of Ni2+ by 53 and 65%, respectively.

Keywords

Trace metal Ethanol production Pichia stipitis Xylose 

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Copyright information

© Society for Industrial Microbiology 2008

Authors and Affiliations

  • Yuan Li
    • 1
  • Zhenya Zhang
    • 1
  • Zhongfang Lei
    • 2
  • Yingnan Yang
    • 1
  • Motoo Utsumi
    • 1
  • Norio Sugiura
    • 1
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Department of Environmental Science and EngineeringFudan UniversityShanghaiChina

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