Production of nisin Z using Lactococcus lactis IO-1 from hydrolyzed sago starch

  • Octavio Carvajal-Zarrabal
  • Cirilo Nolasco-Hipólito
  • Kopli B. Bujang
  • Ayaaki Ishizaki
Original Paper
First Online:
Received:
Accepted:

Abstract

A membrane bioreactor for production of nisin Z was constructed using Lactococcus lactis IO-1 in continuous culture using hydrolyzed sago starch as carbon source. A strategy used to enhance the productivity of nisin Z was to maintain the cells in a continuous growth at high cell concentration. This resulted in a volumetric productivity of nisin Z, as 50,000 IU l−1 h−1 using a cell concentration of 15 g l−1, 30°C, pH 5.5 and a dilution rate of 1.24 h−1. Adding 10 g l−1 YE and 2 g l−1 polypeptone, other inducers were unnecessary to maintain production of nisin. The operating conditions of the reactor removed nisin and lactate, thus minimizing their effects which allowed the maintenance of cells in continuous exponential growth phase mode with high metabolic activity.

Keywords

Nisin Z Bacteriocin Sago starch Membrane bioreactor Lactococcus lactis IO-1 
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References

  1. 1.
    Abriouel H, Valdivia E, Martinez-Bueno M, Maqueda M, Galvez A (2003) A simple method for semi-preparative-scale production and recovery of enterocin AS-48 derived from Enterococcus faecalis subsp. liquefaciens A-48-32. J Microbiol Methods 55:599–605. doi: 10.1016/S0167-7012(03)00202-1 PubMedCrossRefGoogle Scholar
  2. 2.
    Barefoot SF, Klaenhammer TR (1984) Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob Agents Chemother 26:328–334PubMedGoogle Scholar
  3. 3.
    Bhugaloo-Vial P, Grajek W, Dousset X, Boyaval P (1997) Continuous bacteriocin production with high cell density bioreactors. Enzyme Microb Technol 21:450–457. doi: 10.1016/S0141-0229(97)00026-4 CrossRefGoogle Scholar
  4. 4.
    Boyaval P, Bhugaloo-Vial P, Duffes F, Metivier A, Dousset X, Marion D (1998) Production of concentrated bacteriocin solutions in high cell density bioreactors. Lait 78:129–133. doi: 10.1051/lait:1998116 CrossRefGoogle Scholar
  5. 5.
    Chinachoti N, Endo N, Sonomoto K, Ishizaki A (1998) Bioreactor systems for efficient production and separation of nisin Z using Lactococcus lactis IO-1. J Fac Agric Kyushu Univ 42:421–436Google Scholar
  6. 6.
    Chinachoti N, Matsusaki H, Sonomoto K, Ishizaki A (1998) Nisin z production by Lactococcus lactis IO-1 using xylose as a carbon source. Biosci Biotechnol Biochem 62:1022–1024. doi: 10.1271/bbb.62.1022 CrossRefGoogle Scholar
  7. 7.
    De Rojas AH, Martínez B, Suárez JE, Rodríguez A (2004) Enhanced production of lactococcin 972 in chemostat cultures. Appl Microbiol Biotechnol 66:48–52. doi: 10.1007/s00253-004-1661-z PubMedCrossRefGoogle Scholar
  8. 8.
    Desjardins P, Meghrous J, Lacroix C (2001) Effect of aeration and dilution rate on nisin Z production during continuous fermentation with free and immobilized Lactococcus lactis UL719 in supplemented whey permeate. Internal Dairy J 11:943–951. doi: 10.1016/S0958-6946(01)00128-5 CrossRefGoogle Scholar
  9. 9.
    De Vuyst L, Vandamme EJ (1992) Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations. J Gen Microbiol 138:571–578PubMedGoogle Scholar
  10. 10.
    Eijsink VGH, Brurberg MV, Middelhoven PH, Nes IF (1996) Induction of bacteriocin production in Lactobacillus sake by a secreted peptide. J Bacteriol 178:2232–2237PubMedGoogle Scholar
  11. 11.
    Ennahar S, Asou Y, Zendo T, Sonomoto K, Ishizaki A (2001) Biochemical and genetic evidence for production of enterocins A and B by Enterococcus faecium WHE 81. Int J Food Microbiol 70:291–301. doi: 10.1016/S0168-1605(01)00565-7 PubMedCrossRefGoogle Scholar
  12. 12.
    Ishizaki A, Osajima K, Nakamura K, Kimura K, Hara T, Ezaki T (1990) Biochemical characterization of Lactococcus lactis IO-1, whose optimal temperature is as high as 37°C. J Gen Appl Microbiol 36:1–6. doi: 10.2323/jgam.36.1 CrossRefGoogle Scholar
  13. 13.
    Kim WS, Hall RJ, Dunn NW (1997) The effect of nisin concentration and nutrient depletion on nisin production of Lactococcus lactis. Appl Microbiol Biotechnol 48:449–453. doi: 10.1007/s002530051078 PubMedCrossRefGoogle Scholar
  14. 14.
    Matsusaki H, Chinachoti N, Sonomoto K, Ishizaki A (1998) Purification, identification and effective production of a peptide antibiotic produced by Lactococcus lactis IO-1 (JCM 7638). Ann N Y Acad Sci 864:422–427. doi: 10.1111/j.1749-6632.1998.tb10351.x PubMedCrossRefGoogle Scholar
  15. 15.
    Matsusaki H, Endo N, Sonomoto K, Ishizaki A (1996) Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1: relationship between production of the lantibiotic and lactate and cell growth. Appl Microbiol Biotechnol 45:36–40. doi: 10.1007/s002530050645 PubMedCrossRefGoogle Scholar
  16. 16.
    Nilsen T, Nes IF, Holo H (1998) An exported inducer peptide regulates bacteriocin production in Enterococcus faecium ctc492. J Bacteriol 180:1848–1854PubMedGoogle Scholar
  17. 17.
    Nolasco-Hipolito C, Matsunaka T, Kobayashi G, Sonomoto K, Ishizaki A (2002) Synchronized fresh cell bioreactor system for continuous l-(+)-lactic acid production using Lactococcus lactis IO-1 in hydrolyzed sago starch. J Biosci Bioeng 92(3):281–287. doi: 10.1263/jbb.93.281 CrossRefGoogle Scholar
  18. 18.
    Sonomoto K, Chinachoti N, Endo N, Ishizaki A (2000) Biosynthetic production of nisin Z by immobilized Lactococcus lactis IO-1. J Mol Catal, B Enzym 10:325–334. doi: 10.1016/S1381-1177(00)00133-8 CrossRefGoogle Scholar
  19. 19.
    van Belkum MJ, Derksen DJ, Franz CM, Vederas JC (2007) Structure–function relationship of inducer peptide pheromones involved in bacteriocin production in Carnobacterium maltaromaticum and Enterococcus faecium. Microbiology 153:3660–3666. doi: 10.1099/mic.0.2007/009183-0 PubMedCrossRefGoogle Scholar
  20. 20.
    Yoon Y-C, Park H-J, Lee N-K, Paik H-D (2005) Characterization and enhanced production of enterocin HJ35 by Enterococcus faecium HJ35 isolated from human skin. Biotechnol Bioprocess Eng 10:296–303. doi: 10.1007/BF02931845 CrossRefGoogle Scholar

Copyright information

© Society for Industrial Microbiology 2009

Authors and Affiliations

  • Octavio Carvajal-Zarrabal
    • 1
  • Cirilo Nolasco-Hipólito
    • 2
    • 4
  • Kopli B. Bujang
    • 3
  • Ayaaki Ishizaki
    • 2
  1. 1.Instituto de Medicina ForenseUniversidad VeracruzanaBoca del RíoMexico
  2. 2.NECFER Corporation, F-BIC101Kurume-Shi, FukuokaJapan
  3. 3.Deptartment of Molecular BiologyFaculty of Resource Science and Technology, Universiti Malaysia SarawakKota SamarahanMalaysia
  4. 4.R&D Division F-BIC101NECFER CORPORATIONKurume-Shi, FukuokaJapan

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