Journal of Industrial Microbiology & Biotechnology

, Volume 35, Issue 12, pp 1651–1657 | Cite as

Quantitative contributions of bacteria and of Deinococcus geothermalis to deposits and slimes in paper industry

  • Minna Peltola
  • Charlotta Kanto Öqvist
  • Jaakko Ekman
  • Mirva Kosonen
  • Sanna Jokela
  • Marko Kolari
  • Päivi Korhonen
  • Mirja Salkinoja-Salonen
Original Paper
First Online:
Received:
Accepted:

Abstract

Deinococcus geothermalis has frequently been isolated from pink colored deposits of paper industry processes. Laboratory studies have shown that D. geothermalis is capable of forming on nonliving surfaces patchy biofilms that are resistant to adverse agents such as extreme pH, desiccation, solubilising detergents and biocides. This study was done to quantitatively assess the role of D. geothermalis as a biofouler in paper industry. Colored deposits were collected from 24 European and North American paper and board machines and the densities of the bacterial 16S rRNA genes and those of the red slime producers D. geothermalis and Meiothermus spp. were measured by QPCR (quantitative real time PCR). D. geothermalis was found at nine machines, usually from splash area deposits, but its contribution was minor, 0.001–1%, to the total bacterial burden of 8.3 to log 10.5 log units per gram wet-weight of the deposits. When D. geothermalis was found in a measurable quantity, Meiothermus spp. also was found, often in bulk quantity (7–100% of the total bacteria). The data are in line with the properties of D. geothermalis known from laboratory biofilm studies, indicating this species is a pioneer coloniser of machine surfaces and may help other bacteria to adhere and grown into biofilms, rather than competing with them.

Keywords

Biofilm Deinococcus geothermalis Paper machine Biofouling Quantitative RT-PCR 
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Notes

Acknowledgments

This project was financially supported by the Academy of Finland (53305) and the Photobiomics grant (18637) and TEKES Läiskä project (1364/31/05), PhD fellowships and other support from ABS (M.P.) and EnSTe (J.E, C.KÖ) graduate schools are highly acknowledged. We thank the Viikki Science Library and the Faculty Instrument Centre for expert services and Leena Steininger, Hannele Tukiainen and Tuula Suortti for many kinds of help.

References

  1. 1.
    Appling JW, Cruickshank GA, DeLong RF, Herschler RJ, Humiston CG, Martin RB, Sanborn JR, Shema BF, Wiley AJ (1955). Microbiology of pulp and paper. Tappi Monograph series No. 15Google Scholar
  2. 2.
    Claus G, Müller R (1996) Biofilms in a paper mill process water system. In: Heitz E, Flemming HC, Sand W (eds) Microbially influenced corrosion of materials. Springer, Berlin, pp 429–437Google Scholar
  3. 3.
    Costerton JW (2007) The biofilm primer. Springer, Berlin, pp 36–43Google Scholar
  4. 4.
    Daly MJ, Gaidamakova EK, Matrosova VY, Vasilenko A, Zhai M, Venkateswaran A et al (2004) Accumulation of Mn(II) in Deinococcus radiodurans facilitates gamma-radiation resistance. Science 306:1025–1028. doi: 10.1126/science.1103185 PubMedCrossRefGoogle Scholar
  5. 5.
    Edwards U, Rogall T, Blocker H, Emde M, Bottger EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853. doi: 10.1093/nar/17.19.7843 PubMedCrossRefGoogle Scholar
  6. 6.
    Ekman J, Kosonen M, Jokela S, Kolari M, Korhonen P, Salkinoja-Salonen M (2007) Detection and quantitation of colored deposit-forming Meiothermus spp. in paper industry processes and end products. J Ind Microbiol Biotechnol 34:203–211. doi: 10.1007/s10295-006-0187-z PubMedCrossRefGoogle Scholar
  7. 7.
    Ferreira AC, Nobre MF, Rainey FA, Silva MT, Wait R, Burghardt J et al (1997) Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947PubMedCrossRefGoogle Scholar
  8. 8.
    Ghannoum M, O’Toole GA (2004) Microbial biofilms. ASM Press, Washington DC, pp 64–295Google Scholar
  9. 9.
    Hall-Stoodley L, Costerton JW, Stoodley P (2004) Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol 2:95–108. doi: 10.1038/nrmicro821 PubMedCrossRefGoogle Scholar
  10. 10.
    Ingraham JL, Maaløe O, Neidhardt FC (1983) Growth of the bacterial cell. Sinauer Associates Inc., Sunderland, p 3Google Scholar
  11. 11.
    Kanto Öqvist L (2008). Microbial life and deposits in paper machine circuits. PhD thesis, Helsinki University. Dissertations bioscientiarum molecularium Universitatis Helsingiensis Viikki 20/2008 (http://ethesis.helsinki.fi), Helsinki Finland. p 37
  12. 12.
    Kolari M, Nuutinen J, Rainey FA, Salkinoja-Salonen MS (2003) Colored moderately thermophilic bacteria in paper-machine biofilms. J Ind Microbiol Biotechnol 30:225–238. doi: 10.1007/s10295-003-0047-z PubMedGoogle Scholar
  13. 14.
    Kolari M, Nuutinen J, Salkinoja-Salonen MS (2001) Mechanisms of biofilm formation in paper machine by bacillus species: The role of Deinococcus geothermalis. J Ind Microbiol Biotechnol 27:343–351. doi: 10.1038/sj/jim/7000201 PubMedCrossRefGoogle Scholar
  14. 13.
    Kolari M, Schmidt U, Kuismanen E, Salkinoja-Salonen MS (2002) Firm but slippery attachment of Deinococcus geothermalis. J Bacteriol 184:2473–2480. doi: 10.1128/JB.184.9.2473-2480.2002 PubMedCrossRefGoogle Scholar
  15. 15.
    Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar, Buchner A, Lai T, Steppi S, Jobb G, Forster W, Brettske I, Gerber S, Ginhart AW, Gross O, Grumann S, Hermann S, Jost R, Konig A, Liss T, Lussmann R, May M, Nonhoff B, Reichel B, Strehlow R, Stamatakis A, Stuckmann N, Vilbig A, Lenke M, Ludwig T, Bode A, Schleifer KH (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371. doi: 10.1093/nar/gkh293
  16. 16.
    Maaløe O, Kjelgaard NO (1966) Control of macromolecular synthesis. WA Benjamin Inc., New York, p 62Google Scholar
  17. 17.
    Mack D, Davies AP, Harris LG, Rohde H, Horstkotte MA, Knobloch JK (2007) Microbial interactions in Staphylococcus epidermidis biofilms. Anal Bioanal Chem 387:399–408. doi: 10.1007/s00216-006-0745-2 PubMedCrossRefGoogle Scholar
  18. 18.
    Makarova KS, Omelchenko MV, Gaidamakova EK, Matrosova VY, Vasilenko A, Zhai M et al (2007) Deinococcus geothermalis: the pool of extreme radiation resistance genes shrinks. PLoS One 2:e955. doi: 10.1371/journal.pone.0000955
  19. 19.
    Masurat P, Fru EC, Pedersen K (2005) Identification of Meiothermus as the dominant genus in a storage system for spent nuclear fuel. J Appl Microbiol 98:727–740. doi: 10.1111/j.1365-2672.2004.02519.x PubMedCrossRefGoogle Scholar
  20. 20.
    Mattimore V, Battista JR (1996) Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J Bacteriol 178:633–637PubMedGoogle Scholar
  21. 21.
    Nobre MF, da Costa MS (2001) Genus Meiothermus. In: Boone DR, Castenholz RW, Garrity GM (eds) Bergey’s manual of systematic bacteriology, 2nd edn. Springer, BerlinGoogle Scholar
  22. 22.
    Peltola M, Neu TR, Raulio M, Kolari M, Salkinoja-Salonen MS (2008) Architecture of Deinococcus geothermalis biofilms on glass and steel: a lectin study. Environ Microbiol. doi: 10.1111/j.1462-2920.2008.01596.x
  23. 23.
    Pirttijärvi TS, Andersson MA, Salkinoja-Salonen MS (2000) Properties of Bacillus cereus and other bacilli contaminating biomaterial-based industrial processes. Int J Food Microbiol 60:231–239. doi: 10.1016/S0168-1605(00)00313-5 PubMedCrossRefGoogle Scholar
  24. 24.
    Pleckaityte M, Mistiniene E, Michailoviene V, Zvirblis G (2003) Identification and characterization of a Hsp70 (DnaK) chaperone system from Meiothermus ruber. Mol Genet Genomics 269:109–115. doi: 10.1007/s00438-003-0818-2 PubMedGoogle Scholar
  25. 25.
    Priha O, Hallamaa K, Saarela M, Raaska L (2004) Detection of Bacillus cereus group bacteria from cardboard and paper with real-time PCR. J Ind Microbiol Biotechnol 31:161–169. doi: 10.1007/s10295-004-0125-x PubMedCrossRefGoogle Scholar
  26. 26.
    Raulio M, Järn M, Ahola J, Peltonen J, Rosenholm JB, Tervakangas S et al (2008) Microbe repelling coated stainless steel analysed by field emission scanning electron microscopy and physicochemical methods. J Ind Microbiol Biotechnol. doi: 10.1007/s10295-008-0343-8
  27. 27.
    Saarimaa C, Peltola M, Raulio M, Neu TR, Salkinoja-Salonen MS, Neubauer P (2006) Characterization of adhesion threads of Deinococcus geothermalis as type IV pili. J Bacteriol 188:7016–7021. doi: 10.1128/JB.00608-06 PubMedCrossRefGoogle Scholar
  28. 28.
    Sanborn JR (1933) Development and control of microorganisms in a pulp and paper mill system. J Bacteriol 26:373–378PubMedGoogle Scholar
  29. 29.
    Stott MB, Crowe MA, Mountain BW, Smirnova AV, Hou S, Alam M et al (2008) Isolation of novel bacteria, including a candidate division, from geothermal soils in New Zealand. Environ Microbiol. doi: 10.1111/j.1462-2920.2008.01621.x
  30. 30.
    Väisänen OM, Weber A, Bennasar A, Rainey FA, Busse HJ, Salkinoja-Salonen MS (1998) Microbial communities of printing paper machines. J Appl Microbiol 84:1069–1084. doi: 10.1046/j.1365-2672.1998.00447.x PubMedCrossRefGoogle Scholar
  31. 31.
    Väisänen OM, Elo S, Marmo SA, Salkinoja-Salonen M (1989) Enzymatic characterization of Bacilli from food packing paper and board machines. J Ind Microbiol 4:419–428. doi: 10.1007/BF01569637 CrossRefGoogle Scholar
  32. 32.
    Väisänen OM, Mwaisumo NJ, Salkinoja-Salonen MS (1991) Differentiation of dairy strains of the Bacillus cereus group by phage typing, minimum growth temperature, and fatty acid analysis. J Appl Bacteriol 70:315–324PubMedGoogle Scholar

Copyright information

© Society for Industrial Microbiology 2008

Authors and Affiliations

  • Minna Peltola
    • 1
  • Charlotta Kanto Öqvist
    • 1
    • 2
  • Jaakko Ekman
    • 1
  • Mirva Kosonen
    • 4
  • Sanna Jokela
    • 5
  • Marko Kolari
    • 3
  • Päivi Korhonen
    • 6
  • Mirja Salkinoja-Salonen
    • 1
  1. 1.Department of Applied Chemistry and MicrobiologyUniversity of HelsinkiHelsinkiFinland
  2. 2.Ashland Deutschland GmbHEnvironmental and Process SolutionsKrefeldGermany
  3. 3.Kemira Oyj, Kemira Pulp and PaperEspooFinland
  4. 4.Primetieto OyHelsinkiFinland
  5. 5.Finn Spring OySykäräinenFinland
  6. 6.UPM-Kymmene Oyj, Research CenterLappeenrantaFinland

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