Journal of Industrial Microbiology & Biotechnology

, Volume 35, Issue 10, pp 1103–1108 | Cite as

Laccase treatment of recycled blue dyed paper: physical properties and fiber charge

  • Chellandi Mohandass
  • Kristina Knutson
  • Arthur J. Ragauskas
Original Paper


Recycled blue colored paper was treated with laccase under various combinations of physical and chemical parameters including enzyme concentration, temperature, oxygen, and reaction time. Laccase treatment of recycled dyed pulp increased acid group content, tear index, tensile index, and color removal in a dose-dependent manner. Lengthening the treatment time from 2 to 4 h was beneficial to acid group content (12% increase), dye removal, and tensile index but had a detrimental 8% decrease on the tear index. A higher reaction temperature (65 vs. 45 °C) had a beneficial effect on acid group content (+31%), and tensile index (+26%) and a slightly negative effect on tear index (−5%), but significantly reduced the ability of laccase to remove color. Comparison of reactions subjected to different levels of oxygen supplementation showed the greatest beneficial effect for laccase treatment with slow oxygen bubbling. The experimental results indicate that laccase treatment increases fiber carboxylic acid content and tensile strength, in addition to reducing the color of the enzyme treated paper.


Colored paper Carboxylic acid Mediator (TEMPO) Recycling Physical properties Laccase Biobleaching 



We are grateful to Department of Biotechnology, Ministry of Science and Technology, Government of India for funding an overseas Associateship to Dr. C. Mohandass. The authors also wish to thank NSF (EEC-0332554) for their support, in part, of these studies. The technical assistance of Mr. Zheng Deng was highly appreciated.


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Copyright information

© Society for Industrial Microbiology 2008

Authors and Affiliations

  • Chellandi Mohandass
    • 1
  • Kristina Knutson
    • 2
  • Arthur J. Ragauskas
    • 2
  1. 1.Biological Oceanography DivisionNational Institute of OceanographyDona-PaulaIndia
  2. 2.School of Chemistry and Biochemistry, Institute of Paper Science and TechnologyGeorgia Institute of TechnologyAtlantaUSA

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