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Acrylamide synthesis using agar entrapped cells of Rhodococcus rhodochrous PA-34 in a partitioned fed batch reactor

  • Jog Raj
  • Nitya Nand Sharma
  • Shreenath Prasad
  • Tek Chand Bhalla
Original Paper

Abstract

The nitrile hydratase (Nhase) induced cells of Rhodococcus rhodochrous PA-34 catalyzed the conversion of acrylonitrile to acrylamide. The cells of R. rhodochrous PA-34 immobilized in 2% (w/v) agar (1.76 mg dcw/ml agar matrix) exhibited maximum Nhase activity (8.25 U/mg dcw) for conversion of acrylonitrile to acrylamide at 10°C in the reaction mixture containing 0.1 M potassium phosphate buffer (pH 7.5), 8% (w/v) acrylonitrile and immobilized cells equivalent to 1.12 mg dcw (dry cell weight) per ml. In a partitioned fed batch reaction at 10°C, using 1.12 g dcw immobilized cells in a final volume of 1 l, a total of 372 g of acrylonitrile was completely hydrated to acrylamide (498 g) in 24 h. From the above reaction mixture 87% acrylamide (432 g) was recovered through crystallization at 4°C. By recycling the immobilized biocatalyst (six times), a total of 2,115 g acrylamide was produced.

Keywords

Rhodococcus rhodochrous PA-34 Immobilized biocatalyst Agar Acrylonitrile Acrylamide Partitioned reactor 

Notes

Acknowledgments

We acknowledge the financial support in the form of Senior Research Fellowship from University Grants Commission (UGC), New Delhi, India to Mr. Jog Raj, from the Council and Scientific and Industrial Research (CSIR) to Mr. Nitya Nand Sharma and from the Department of Biotechnology, Government of India, New Delhi to Mr. Shreenath Prasad. The computational facility availed at Bioinformatics Centre, H. P. University is gratefully acknowledge.

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Copyright information

© Society for Industrial Microbiology 2007

Authors and Affiliations

  • Jog Raj
    • 1
  • Nitya Nand Sharma
    • 1
  • Shreenath Prasad
    • 1
  • Tek Chand Bhalla
    • 1
  1. 1.Department of BiotechnologyHimachal Pradesh UniversityShimlaIndia

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