Production of ethanol from corn stover hemicellulose hydrolyzate using Pichia stipitis
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Hemicellulose liquid hydrolyzate from dilute acid pretreated corn stover was fermented to ethanol using Pichia stipitis CBS 6054. The fermentation rate increased with aeration but the pH also increased due to consumption of acetic acid by Pichia stipitis. Hemicellulose hydrolyzate containing 34 g/L xylose, 8 g/L glucose, 8 g/L Acetic acid, 0.73 g/L furfural, and 1 g/L hydroxymethyl furfural was fermented to 15 g/L ethanol in 72 h. The yield in all the hemicellulose hydrolyzates was 0.37–0.44 g ethanol/g (glucose + xylose). Nondetoxified hemicellulose hydrolyzate from dilute acid pretreated corn stover was fermented to ethanol with high yields, and this has the potential to improve the economics of the biomass to ethanol process.
KeywordsAcetic acid Pretreatment Inhibitors Ethanol Fermentation Biomass Xylose
This investigation was supported by the Abengoa-DOE project. We like to thank Dr. Thomas Jeffries for generously providing the yeast strain, David Milam for analytical support on HPLC and Dr. K. C. McFarland for HMF and furfural analyses. We also like to thank Dr. Guillermo Coward-Kelly, Dr. Mads Torry-Smith and Dr. Frank D. Haagensen for useful discussions.
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