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Visualization of yeast single-cells on fabric surface with a fluorescent glucose and their isolation for culture

  • Kohtaro Fujioka
  • Philip Geis
  • Mikako Saito
  • Hideaki MatsuokaEmail author
Short Communication

Abstract

An ultra-deep focusing range (UDF) fluorescent microscope system has been combined with a micromanipulation system to develop a viable cell detection-identification system applicable to microbes on environmental surfaces and products. Candida albicans yeast cells on a fabric sample surface were viably stained with a fluorescent glucose derivative, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy glucose (2-NBDG) and detected with a UDF fluorescent microscope. Visualized single-cells of C. albicans were picked in a glass microcapillary and transferred onto an agar medium. After the culture, the colony was assayed for DNA sequence to identify the isolate. This demonstrates a potential application to the study of unknown environmental microorganisms.

Keywords

Ultra-deep focusing range (UDF) fluorescent microscope Single-cell manipulation Fluorescent glucose derivative Viable cell imaging 

Notes

Acknowledgments

This research was supported by the Microbial Visualization Community of Practice in The Procter & Gamble Company. H.M. acknowledges supports from Grant-in-aid for Scientific Research for the Promotion of Safety and Security of Foods, The Ministry of Health, Labor, and Welfare, and from CREST of Japan Science and Technology Agency.

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Copyright information

© Society for Industrial Microbiology 2007

Authors and Affiliations

  • Kohtaro Fujioka
    • 1
    • 2
  • Philip Geis
    • 3
  • Mikako Saito
    • 2
  • Hideaki Matsuoka
    • 2
    Email author
  1. 1.Kobe Technical CenterProcter & Gamble Far East, Inc.KobeJapan
  2. 2.Department of Biotechnology and Life ScienceTokyo University of Agriculture and TechnologyTokyoJapan
  3. 3.Sharon Woods Technical CenterThe Procter & Gamble Co.CincinnatiUSA

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