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Isolation and partial characterization of antimicrobial compounds from a new strain Nonomuraea sp. NM94

  • Boubekeur Badji
  • Abdellah Mostefaoui
  • Nasserdine Sabaou
  • Ahmed Lebrihi
  • Florence Mathieu
  • Elisabeth Seguin
  • François Tillequin
Original Paper

Abstract

An actinomycete strain NM94 was isolated from a Saharan soil sample by a dilution agar plating method using chitin-vitamins B medium supplemented with penicillin. The strain presented the morphological and chemical characteristics of the genus Nonomuraea. On the basis of 16S rDNA analysis and physiological tests, this isolate was found to be quite different from the known species of Nonomuraea and might be new. The strain NM94 secreted several antibiotics on yeast extract malt extract glucose medium that were active against some Gram-positive bacteria, yeast, and fungi. The antibiotics were extracted with dichloromethane and detected by bioautography on silica gel plates using Mucor ramannianus and Bacillus subtilis as the test organisms. Among these antibiotics, a complex called 94A showed interesting antifungal activity. It was selected and purified by reverse-phase HPLC. This complex was composed of five compounds. Spectroscopic studies by infrared, mass, and 1H NMR of the compounds were carried out. Initial results showed that these molecules differed from the known antibiotics produced by other Nonomuraea species.

Keywords

Actinomycetes Nonomuraea Taxonomy Antifungals Saharan soil 

Notes

Acknowledgments

This work was gratefully supported by the Comité d’Evaluation et de Prospective de Coopération Interuniversitaire Franco-Algérienne (CMEP No 02 MDU 564), French Embassy in Algiers. The authors wish to thank L. Marchal (Service de Microscopie, Faculté de Médecine de Nancy, France) for electron microscopy.

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Copyright information

© Society for Industrial Microbiology 2007

Authors and Affiliations

  • Boubekeur Badji
    • 1
  • Abdellah Mostefaoui
    • 2
  • Nasserdine Sabaou
    • 1
  • Ahmed Lebrihi
    • 3
  • Florence Mathieu
    • 3
  • Elisabeth Seguin
    • 4
  • François Tillequin
    • 5
  1. 1.Laboratoire de Recherche sur les Produits Bioactifs et la Valorisation de la BiomasseEcole Normale Supérieure de KoubaVieux-Kouba, AlgerAlgérie
  2. 2.Département de BiologieUniversité de DjelfaDjelfaAlgérie
  3. 3.Ecole Nationale Supérieure Agronomique de Toulouse, INPT, Laboratoire de Génie chimique, UMR 5503Castanet-Tolosan Cedex 1France
  4. 4.Laboratoire de PharmacognosieUniversité de Rouen-Haute Normandie, Faculté de PharmacieRouen Cedex 1France
  5. 5.Laboratoire de Pharmacognosie de l’Université René Descartes, UMR/CNRS 8638, Faculté des Sciences Pharmaceutiques et BiologiquesParisFrance

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