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A recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli

  • Daniel Manderson
  • Robert Dempster
  • Yusuf ChistiEmail author
Original Paper

Abstract

A commercial process was developed for producing a recombinant vaccine against hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the oncospheres of the hydatid worm (Echinococcus granulosus), was produced as inclusion bodies in Escherichia coli. Fed-batch cultures of E. coli using Terrific broth in stirred bioreactors at 37°C, pH 7.0, and a dissolved oxygen level of 30% of air saturation produced the highest volumetric concentrations of the final solubilized antigen. An exponential feeding strategy proved distinctly superior to feeding based on pH-stat and DO-stat methods. The plasmid coding for the antigen was induced with isopropyl-β-D-thiogalactopyranoside (IPTG) at 4 h after initiation of the culture. The minimum IPTG concentration for full induction was 0.1 mM.

Keywords

Echinococcus granulosus Escherichia coli Hydatid Hydatidosis Recombinant vaccine Veterinary vaccine 

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Copyright information

© Society for Industrial Microbiology 2005

Authors and Affiliations

  • Daniel Manderson
    • 1
  • Robert Dempster
    • 2
  • Yusuf Chisti
    • 1
    Email author
  1. 1.Institute of Technology and Engineering PN456Massey UniversityPalmerston NorthNew Zealand
  2. 2.AgResearch LtdWallaceville Animal Research CenterUpper HuttNew Zealand

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