Anaerobic co-reduction of chromate and nitrate by bacterial cultures of Staphylococcus epidermidis L-02

  • A Vatsouria
  • M Vainshtein
  • P Kuschk
  • A Wiessner
  • Kosolapov D
  • M Kaestner
Original Paper

Abstract

Industrial wastewater is often polluted by Cr(VI) compounds, presenting a serious environmental problem. This study addresses the removal of toxic, mutagenic Cr(VI) by means of microbial reduction to Cr(III), which can then be precipitated as oxides or hydroxides and extracted from the aquatic system. A strain of Staphylococcus epidermidis L-02 was isolated from a bacterial consortium used for the remediation of a chromate-contaminated constructed wetland system. This strain reduced Cr(VI) by using pyruvate as an electron donor under anaerobic conditions. The aims of the present study were to investigate the specific rate of Cr(VI) reduction by the strain L-02, the effects of chromate and nitrate (available as electron acceptors) on the strain, and the interference of chromate and nitrate reduction processes. The presence of Cr(VI) decreased the growth rate of the bacterium. Chromate and nitrate reduction did not occur under sterile conditions but was observed during tests with the strain L-02. The presence of nitrate increased both the specific Cr(VI) reduction rate and the cell number. Under denitrifying conditions, Cr(VI) reduction was not inhibited by nitrite, which was produced during nitrate reduction. The average specific rate of chromate reduction reached 4.4 μmol Cr 1010 cells−1 h−1, but was only 2.0 μmol Cr 1010 cells−1 h−1 at 20 °C. The maximum specific rate was as high as 8.8–9.8 μmol Cr 1010 cells−1 h−1. The role of nitrate in chromate reduction is discussed.

Keywords

Chromate reduction Nitrate reduction Bacteria Staphylococcus epidermidis 

Notes

Acknowledgement

The research was kindly supported by the Linkage NATO grant EST-CLG-978918.

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Copyright information

© Society for Industrial Microbiology 2005

Authors and Affiliations

  • A Vatsouria
    • 1
  • M Vainshtein
    • 1
  • P Kuschk
    • 2
  • A Wiessner
    • 2
  • Kosolapov D
    • 3
  • M Kaestner
    • 2
  1. 1.Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchinoRussia
  2. 2.Department of BioremediationUFZ Centre for Environmental Research Leipzig-Halle GmbHLeipzigGermany
  3. 3.Institute for Biology of Inland WatersRussian Academy of SciencesYaroslavlRussia

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