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Evaluation of Streptomyces sp. strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets

  • K. Getha
  • S. Vikineswary
  • W. H. Wong
  • T. Seki
  • A. Ward
  • M. Goodfellow
Original Paper

Abstract

Streptomyces sp. strain g10 exhibited strong antagonism towards Fusarium oxysporum f.sp. cubense (Foc) races 1, 2 and 4 in plate assays by producing extracellular antifungal metabolites. Treating the planting hole and roots of 4-week-old tissue-culture-derived ‘Novaria’ banana plantlets with strain g10 suspension (108 cfu/ml), significantly (P<0.05) reduced wilt severity when the plantlets were inoculated with 104 spores/ml Foc race 4. The final disease severity index for leaf symptom (LSI) and rhizome discoloration (RDI) was reduced about 47 and 53%, respectively, in strain g10-treated plantlets compared to untreated plantlets. Reduction in disease incidence was not significant (P<0.05) when plantlets were inoculated with a higher concentration (106 spores/ml) of Foc race 4. Rhizosphere population of strain g10 showed significant (P=0.05) increase of more than 2-fold at the end of the 3rd week compared to the 2nd week after soil amendment with the antagonist. Although the level dropped, the rhizosphere population at the end of the 6th week was still nearly 2-fold higher than the level detected after 2 weeks. In contrast, the root-free population declined significantly (P=0.05), nearly 4-fold after 6 weeks when compared to the level detected after 2 weeks. Neither growth-inhibiting nor growth-stimulating effects were observed in plantlets grown in strain g10-amended soil.

Keywords

Actinomycete biocontrol agent Fusarium oxysporum f.sp. cubense Fusarium wilt control Streptomyces violaceusniger Rhizosphere 

Notes

Acknowledgements

This study was supported by IRPA grant no. 08-02-03-0144 from the Ministry of Science, Technology and the Environment, Malaysia. We gratefully thank the Japanese Society for Promotion of Science, Japan and British Council, UK for facilitating the collaborations. We also thank University Malaya for their generous support.

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Copyright information

© Society for Industrial Microbiology 2005

Authors and Affiliations

  • K. Getha
    • 1
  • S. Vikineswary
    • 2
  • W. H. Wong
    • 3
  • T. Seki
    • 4
  • A. Ward
    • 5
  • M. Goodfellow
    • 5
  1. 1.Forest Research InstituteKuala LumpurMalaysia
  2. 2.Institute of Biological SciencesUniversity MalayaKuala LumpurMalaysia
  3. 3.Sepang Institute of TechnologyKlang, Selangor Darul EhsanMalaysia
  4. 4.International Center for BiotechnologyOsaka UniversityOsakaJapan
  5. 5.Department of Agricultural and Environmental ScienceUniversity of NewcastleUK

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