Characterization of thermostable Xyn10A enzyme from mesophilic Clostridium acetobutylicum ATCC 824

  • Mursheda K. Ali
  • Frederick B. Rudolph
  • George N. BennettEmail author
Original Paper


A thermostable xylanase gene, xyn10A (CAP0053), was cloned from Clostridium acetobutylicum ATCC 824. The nucleotide sequence of the C. acetobutylicum xyn10A gene encoded a 318-amino-acid, single-domain, family 10 xylanase, Xyn10A, with a molecular mass of 34 kDa. Xyn10A exhibited extremely high (92%) amino acid sequence identity with Xyn10B (CAP0116) of this strain and had 42% and 32% identity with the catalytic domains of Rhodothermus marinus xylanase I and Thermoascus aurantiacus xylanase I, respectively. Xyn10A enzyme was purified from recombinant Escherichia coli and was highly active toward oat-spelt and Birchwood xylan and slightly active toward carboxymethyl cellulose, arabinogalactouronic acid, and various p-nitrophenyl monosaccharides. Xyn10A hydrolyzed xylan and xylooligosaccharides larger than xylobiose to produce xylose. This enzyme was optimally active at 60°C and had an optimum pH of 5.0. This is one of a number of related activities encoded on the large plasmid in this strain.


Xylanase Clostridium acetobutylicum Biomass Thermostable Hemicellulose Xylose 



We thank Prof. Kazuo Sakka, Mie University, Japan, for supplying pQE-30T. This research was supported by the United States Department of Agriculture, Grant 00-35500-926, and the Robert A. Welch Foundation, grants C-1268 and C-1372.


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Copyright information

© Society for Industrial Microbiology 2005

Authors and Affiliations

  • Mursheda K. Ali
    • 1
  • Frederick B. Rudolph
    • 1
  • George N. Bennett
    • 1
    Email author
  1. 1.Department of Biochemistry and Cell BiologyRice UniversityHoustonUSA

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