Sequences affecting the regulation of solvent production in Clostridium acetobutylicum

  • Miles C. Scotcher
  • Ke-xue Huang
  • Mary L. Harrison
  • Frederick B. Rudolph
  • George N. Bennett
Original Paper

DOI: 10.1007/s10295-003-0057-x

Cite this article as:
Scotcher, M.C., Huang, K., Harrison, M.L. et al. J IND MICROBIOL BIOTECHNOL (2003) 30: 414. doi:10.1007/s10295-003-0057-x

Abstract

The high solvent phenotype of Clostridium acetobutylicum mutants B and H was complemented by the introduction of a plasmid that contains either an intact or partially-deleted copy of solR, restoring acetone and butanol production to wild-type levels. This demonstrates that the solR open reading frame on pSOLThi is not required to restore solvent levels. The promoter region upstream of alcohol dehydrogense E (adhE) was examined in efforts to identify sites that play major roles in the control of expression. A series of adhE promoter fragments was constructed and the expression of each in acid- and solvent-phases of growth was analyzed using a chloramphenicol acetyl-transferase reporter system. Our results show that a region beyond the 0A box is needed for full induction of the promoter. Additionally, we show that the presence of sequences around a possible processing site designated S2 may have a negative role in the regulation of adhE expression.

Keywords

Clostridium Solvent Sol operon CAT reporter Alcohol dehydrogenase E 

Copyright information

© Society for Industrial Microbiology 2003

Authors and Affiliations

  • Miles C. Scotcher
    • 1
  • Ke-xue Huang
    • 1
    • 2
  • Mary L. Harrison
    • 1
  • Frederick B. Rudolph
    • 1
  • George N. Bennett
    • 1
  1. 1.Department of Biochemistry and Cell Biology, MS 140Rice UniversityHoustonUSA
  2. 2.Dow Agrosciences LLCR & D DiscoveryIndianapolisUSA

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