, Volume 53, Issue 6, pp 427–432 | Cite as

Survival of anaerobic fungus Caecomyces sp. in various preservation methods: a comparative study

  • Ravinder Nagpal
  • Anil Kumar Puniya
  • Jatinder Paul Sehgal
  • Kishan Singh
Full Paper


The present investigation was designed to observe the survival of the anaerobic fungus Caecomyces sp. in various routine preservation methods. Among all the treatments, cryopreservation of fungi at −70 °C with glycerol was found to be most effective for long-term maintenance (more than 90 days) of rumen fungi, followed by dimethyl sulfoxide (DMSO) and ethylene glycol (up to 60 days). In contrast, at −196 °C, DMSO showed maximum survival (more than 90 days), followed by glycerol (up to 90 days) and ethylene glycol (up to 30 days). At 39 °C, maximum survival (up to 30 days) was observed with soft agar and wheat straw; at refrigeration temperature, preservation with Orpin’s media containing straw showed maximum survival (up to 30 days).


Anaerobic fungi Cryopreservation Fiber degradation Microbial survival Rumen microflora 



The authors are thankful for the research fellowships and funding provided by National Dairy Research Institute, Karnal, and Indian Council of Agricultural Research, New Delhi, India.


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Copyright information

© The Mycological Society of Japan and Springer 2012

Authors and Affiliations

  • Ravinder Nagpal
    • 1
  • Anil Kumar Puniya
    • 1
  • Jatinder Paul Sehgal
    • 2
  • Kishan Singh
    • 1
  1. 1.Dairy Microbiology DivisionNational Dairy Research InstituteKarnalIndia
  2. 2.Dairy Cattle Nutrition DivisionNational Dairy Research InstituteKarnalIndia

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