, Volume 51, Issue 2, pp 134–138 | Cite as

Effect of a hot water-soluble extraction from Grifola frondosa on the viability of a human monocyte cell line exposed to mitomycin C

  • Noriko KodamaEmail author
  • Shigeto Mizuno
  • Akihiro Asakawa
  • Akio Inui
  • Hiroaki Nanba
Short Communication


The effects of a hot water-soluble extract of Grifola frondosa on the viability of multiple immune cell lines were examined in vitro. The extract and the 1000-cut extract, a low molecular weight fraction, improved the viability of a human monocyte cell line, THP-1, treated by the anticancer drug mitomycin C. DNA microarray and immunoassay results revealed increases in interleukin (IL)-8 expression by THP-1 cells in response to the 1000-cut extract. These data suggest that the extract would enhance the immune system through induction of IL-8 production by human blood monocytes, which leads to activated neutrophils.


Maitake mushroom IL-8 Extract Viability 


  1. Adachi K, Nanba H, Kuroda H (1987) Potentiation of host-mediated antitumor activity in mice by β-glucan obtained from Grifola frondosa (maitake). Chem Pharm Bull 35:262–270PubMedGoogle Scholar
  2. Asnis GM, De La Garza R 2nd (2006) Interferon-induced depression in chronic hepatitis C: a review of its prevalence, risk factors, biology, and treatment approaches. J Clin Gastroenterol 40:322–335CrossRefPubMedGoogle Scholar
  3. Borchers AT, Stern JS, Hackman RM, Keen CL, Gershwin ME (1999) Tumors, and immunity. Proc Soc Exp Biol Med 221:281–293CrossRefPubMedGoogle Scholar
  4. Ellertsen LK, Hetland G, Johnson E, Grinde B (2006) Effect of a medicinal extract from Agaricus blazei Murill on gene expression in a human monocyte cell line as examined by microarray and immuno assays. Int Immunopharmacol 6:133–143CrossRefPubMedGoogle Scholar
  5. Harada N, Kodama N, Nanba H (2003) Relationship between dendritic cells and the D-fraction-induced Th-1 dominant response in BALB/c tumor-bearing mice. Cancer Lett 192:181–187CrossRefPubMedGoogle Scholar
  6. Hurwitz H (2004) Integrating the anti-VEGF-A humanized monoclonal antibody bevacizumab with chemotherapy in advanced colorectal cancer. Clin Colorectal Cancer 2:S62–S68CrossRefGoogle Scholar
  7. Illman J, Corringham R, Robinson D Jr, Davis HM, Rossi JF, Cella D, Trikha M (2005) Are inflammatory cytokines the common link between cancer-associated cachexia and depression? J Support Oncol 3:37–50PubMedGoogle Scholar
  8. Inoue A, Kodama N, Nanba H (2002) Effects of maitake (Grifola frondosa) D-fraction on the control of the T lymph node Th-1/Th-2 proportion. Biol Pharm Bull 25:536–540CrossRefPubMedGoogle Scholar
  9. Kodama N, Harada N, Nanba H (2002a) A polysaccharide, extract from Grifola frondosa, induces Th-1 dominant response in carcinoma-bearing BALB/c mice. Jpn J Pharmacol 90:357–360CrossRefPubMedGoogle Scholar
  10. Kodama N, Kumuta K, Nanba H (2002b) Effect of D-fraction, a polysaccharide from Grifola frondosa on tumor growth involve activation of NK cells. Biol Pharm Bull 25:1642–1645CrossRefGoogle Scholar
  11. Kodama N, Murata Y, Asakawa A, Inui A, Hayashi M, Sakai N, Nanba H (2005) Maitake D-fraction enhances antitumor effects and reduces immunosuppression by mitomycin-C in tumor-bearing mice. Nutrition 21:624–629CrossRefPubMedGoogle Scholar
  12. Li H, Cao MY, Lee Y, Nenatar T, Lee Y, Feng N (2007) Virulizin, a novel immunotherapy agent, stimulates TNF-α expression in monocytes/macrophages in vitro and in vivo. Int Immunopharmacol 7:1350–1359CrossRefPubMedGoogle Scholar
  13. Mukaida N, Harada A, Matsushima K (1995) A novel leukocyte chemotactic and activating cytokine, interleukin-8 (IL-8). Cancer Treat Res 80:261–286PubMedGoogle Scholar
  14. Nanba H, Hamaguchi A, Kuroda H (1987) The chemical structure of an antitumor polysaccharide in fruit bodies of Grifola frondosa (Maitake). Chem Pharm Bull 35:1162–1168PubMedGoogle Scholar
  15. Saji S, Sugiyama Y, Kunieda K, Umemoto T (1999) Recent progress in biological therapies for cancer. Gan to Kagaku Ryoho 1:32–41 (in Japanese)Google Scholar
  16. Shibata M, Nezu T, Fujisaki S, Andou K, Tomita R, Fukuzawa M (2002) Clinical potential of biological response modifiers combined with chemotherapy for gastric cancer. Japanese experience. Dig Surg 19:255–260CrossRefPubMedGoogle Scholar
  17. Urushizaki I (1989) Therapy by biological response modifiers. Rinsho Ketsueki 30:1230–1232 (in Japanese)PubMedGoogle Scholar

Copyright information

© The Mycological Society of Japan and Springer 2010

Authors and Affiliations

  • Noriko Kodama
    • 1
    Email author
  • Shigeto Mizuno
    • 2
  • Akihiro Asakawa
    • 3
  • Akio Inui
    • 3
  • Hiroaki Nanba
    • 4
  1. 1.Department of Basic Educational Center for PharmacyKobe Pharmaceutical UniversityKobeJapan
  2. 2.Department of Medical PharmaceuticsKobe Pharmaceutical UniversityKobeJapan
  3. 3.Department of Behavioral MedicineKagoshima University Graduate School of Medical and Dental SciencesKagoshimaJapan
  4. 4.Kobe Pharmaceutical UniversityKobeJapan

Personalised recommendations