Advertisement

Mycoscience

, Volume 44, Issue 3, pp 197–202 | Cite as

Phylogenetic position of Pholiota nameko in the genus Pholiota inferred from restriction analysis of ribosomal DNA

  • Teruyuki Matsumoto
  • Yasushi Obatake
  • Yukitaka Fukumasa-Nakai
  • Eiji Nagasawa
FULL PAPER

Abstract

 To estimate the phylogenetic position of Pholiota nameko in the genus Pholiota, restriction fragment length polymorphisms (RFLPs) for PCR products of 26S ribosomal RNA gene (rDNA), internal transcribed spacers (ITS), and intergenic spacer (IGS) of the rDNA repeat from P. nameko and eight of its closely related species were investigated, and a phylogenetic tree was constructed based on data that resulted from RFLP analysis. P. nameko was clustered together with P. adiposa, P. limonella, and P. aurivella of the subgenus Pholiota (section Adiposae). However, P. nameko and P. albocrenulata, both of which belong to the subgenus Hemipholiota, were phylogenetically separated from each other. Our results suggested that P. nameko is closely related to the members of the section Adiposae. Furthermore, the phylogenetic distance between this section Adiposae group including P. nameko and Kuehneromyces mutabillis was smaller than that between P. malicola var. macropoda of the subgenus Flammula and the members of section Adiposae. Our data indicate that molecular information on rDNA will be useful to reconstruct taxa within the genus Pholiota in the family Strophariacea that have been classified mostly on the basis of morphological characters.

Key words Intergenic spacer (IGS) Internal transcribed spacer (ITS) PCR-RFLP Phylogeny 26S rDNA 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Tokyo 2003

Authors and Affiliations

  • Teruyuki Matsumoto
    • 1
  • Yasushi Obatake
    • 2
  • Yukitaka Fukumasa-Nakai
    • 1
  • Eiji Nagasawa
    • 1
  1. 1.The Tottori Mycological Institute, Kokoge 211, Tottori 689-1125, Japan Tel. +81-857-51-8111; Fax +81-857-53-1986 e-mail: kin-matu@infosakyu.ne.jpJP
  2. 2.Nara Forest Research Institute, Nara, JapanJP

Personalised recommendations