, Volume 103, Issue 2, pp 126–135 | Cite as

Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances

  • Tomoyuki Kawase
Review Article


Over the past decade, platelet-rich plasma (PRP), a platelet-concentrated plasma fraction, has been widely investigated and applied to regenerative medicine. The clinical utility of PRP is supported by evidence that PRP contains high concentrations of platelet-related growth factors and normal concentrations of plasma-derived fibrinogen, both of which contribute synergistically to the regenerative process. Additionally, its superior cost-efficacy versus conventional therapies is attractive to many clinicians. However, current disadvantages of PRP include a relatively complicated preparation procedure and variable operator-dependent efficacy. An additional disadvantage is the use of bovine thrombin, an animal-derived biological, as a coagulant. Many of these disadvantages are overcome by recent advances in preparation procedures and devices; for example, Joseph Choukroun simplified the platelet-rich fibrin preparation procedure and improved handling efficiency without the aid of animal-derived factors. With advancements in cell processing technology, there has been a general shift in cell therapy from autologous to allogeneic treatment; however, autologous PRP therapy will not easily be replaced by allogeneic treatment in the near future. Therefore, to provide more predictable regenerative therapy outcomes using autologous PRP, further investigations should address developing a standardized procedure for PRP preparation to augment its efficacy and potency, independent of donor variability. We would then propose that operators and clinicians prepare PRP according to the standardized protocol and to carefully evaluate the clinical scenario (i.e., recipient factors comprising skeletal defects) to determine which factor(s) should be added to PRP preparations. This careful approach will lead to improved clinical outcomes for patients.


Platelet-rich plasma Platelet-rich fibrin Quality control Standardization Regenerative medicine 



The author expresses special thanks to Prof. Kazuhiro Okuda (Department of Periodontology, Institute of Medicine and Dentistry, Niigata University), Prof. Takaaki Tanaka (Department of Materials Science and Technology, Niigata University), and Prof. Larry F. Wolff (Department of Periodontology, University of Minnesota School of Dentistry) for their helpful comments on this article. This project was financially supported by a Grant-in-Aid for scientific research from JSPS KAKENHI Grant Numbers 24390443 and 24390465.

Conflict of interest

The author declares no competing financial interests.


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Copyright information

© The Society of The Nippon Dental University 2015

Authors and Affiliations

  1. 1.Division of Oral Bioengineering, Institute of Medicine and DentistryNiigata UniversityNiigataJapan
  2. 2.Advanced Research CenterThe Nippon Dental University School of Life Dentistry at NiigataNiigataJapan

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