Cell wall Glycine-rich Protein2 is involved in tapetal differentiation and pollen maturation

Journal of Plant Research volume 133, pages883–895(2020)Cite this article

Abstract

The tapetum plays important roles in anther development by providing materials for pollen-wall formation and nutrients for pollen development. Here, we report the characterization of a male-sterile mutant of glycine-rich protein 2 (OsGRP2), which exhibits irregular cell division and dysfunction of the tapetum. GRP is a cellwall structural protein present in the cell walls of diverse plant species, but its function is unclear in pollen development. We found that few GRP genes are expressed in rice and thus focused on one highly expressed gene, OsGRP2. The tapetal cell walls of an OsGRP2 mutant did not thicken at the pollen mothercell stage, as a result, pollen maturation and fertility rate decreased. High OsGRP2 expression was detected in male-floral organs, and OsGRP2 was distributed in the tapetum. OsGRP2 participated in establishment of the cellwall network during early tapetum development. In conclusion, our results indicate that OsGRP2 plays important roles in the differentiation and function of the tapetum.

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Acknowledgements

This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (18075004 to H. Iwai), a Grant-in-Aid for Scientific Research on Innovative Areas (24114006 to S. Satoh and H. Iwai).

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  1. Naomi Takebe and Hiroaki Iwai contributed equally to this work.

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  1. Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Tsukuba, 305-8572, Japan

    Naomi Takebe, Atsuko Nakamura, Tomomi Watanabe, Aya Miyashita, Shinobu Satoh & Hiroaki Iwai

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  1. Naomi Takebe
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  2. Atsuko Nakamura
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Correspondence to Hiroaki Iwai.

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Takebe, N., Nakamura, A., Watanabe, T. et al. Cell wall Glycine-rich Protein2 is involved in tapetal differentiation and pollen maturation. J Plant Res 133, 883–895 (2020). https://doi.org/10.1007/s10265-020-01223-x

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Keywords

  • Cell wall
  • Glycine-rich protein
  • Male sterility
  • Oryza sativa
  • Pollen
  • Tapetum