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Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens

  • Ryo Furukawa
  • Michiki Aso
  • Tomomichi Fujita
  • Seiji Akimoto
  • Ryouichi Tanaka
  • Ayumi Tanaka
  • Makio YokonoEmail author
  • Atsushi Takabayashi
Regular Paper

Abstract

Mosses are one of the earliest land plants that diverged from fresh-water green algae. They are considered to have acquired a higher capacity for thermal energy dissipation to cope with dynamically changing solar irradiance by utilizing both the “algal-type” light-harvesting complex stress-related (LHCSR)-dependent and the “plant-type” PsbS-dependent mechanisms. It is hypothesized that the formation of photosystem (PS) I and II megacomplex is another mechanism to protect photosynthetic machinery from strong irradiance. Herein, we describe the analysis of the PSI–PSII megacomplex from the model moss, Physcomitrella patens, which was resolved using large-pore clear-native polyacrylamide gel electrophoresis (lpCN-PAGE). The similarity in the migration distance of the Physcomitrella PSI–PSII megacomplex to the Arabidopsis megacomplex shown during lpCN-PAGE suggested that the Physcomitrella PSI–PSII and Arabidopsis megacomplexes have similar structures. Time-resolved chlorophyll fluorescence measurements show that excitation energy was rapidly and efficiently transferred from PSII to PSI, providing evidence of an ordered association of the two photosystems. We also found that LHCSR and PsbS co-migrated with the Physcomitrella PSI–PSII megacomplex. The megacomplex showed pH-dependent chlorophyll fluorescence quenching, which may have been induced by LHCSR and/or PsbS proteins with the collaboration of zeaxanthin. We discuss the mechanism that regulates the energy distribution balance between two photosystems in Physcomitrella.

Keywords

Physcomitrella PSI–PSII megacomplex LHCSR CN-PAGE 

Notes

Acknowledgements

This work was supported by JSPS (Japan Society for the Promotion of Science) KAKENHI Grant numbers 23770035 to A. Takabayashi, 16H06553 to S. Akimoto, and 16H06554 to R. Tanaka.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10265_2019_1138_MOESM1_ESM.pdf (7.4 mb)
Supplementary material 1 (PDF 7591 kb)

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Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Low-Temperature ScienceHokkaido UniversitySapporoJapan
  2. 2.Faculty of ScienceHokkaido UniversitySapporoJapan
  3. 3.Graduate School of ScienceKobe UniversityKobeJapan
  4. 4.Innovation CenterNippon Flour Mills Co., Ltd.AtsugiJapan

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