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Journal of Plant Research

, Volume 132, Issue 4, pp 461–471 | Cite as

Nicotiana suaveolens accessions with different ploidy levels exhibit different reproductive isolation mechanisms in interspecific crosses with Nicotiana tabacum

  • Hai He
  • Takahiro Iizuka
  • Maho Maekawa
  • Kumi Sadahisa
  • Toshinobu Morikawa
  • Masanori Yanase
  • Shuji Yokoi
  • Masayuki Oda
  • Takahiro TezukaEmail author
Regular Paper

Abstract

Reproductive isolation, including prezygotic and postzygotic barriers, is a mechanism that separates species. Many species in the Nicotiana section Suaveolentes exhibit reproductive isolation in crosses with Nicotiana tabacum. In this study, we investigated whether the chromosome numbers and ploidy levels of eight Nicotiana suaveolens accessions are related to the reproductive isolation after crosses with N. tabacum by flow cytometry and chromosome analyses. Additionally, the internal transcribed spacer (ITS) regions of the eight N. suaveolens accessions were sequenced and compared with the previously reported sequences of 22 Suaveolentes species to elucidate the phylogenetic relationships in the section Suaveolentes. We revealed that four N. suaveolens accessions comprised 64 chromosomes, while the other four accessions carried 32 chromosomes. Depending on the ploidy levels of N. suaveolens, several types of reproductive isolation were observed after crosses with N. tabacum, including decreases in the number of capsules and the germination rates of hybrid seeds, as well as hybrid lethality and abscission of enlarged ovaries at 12–17 days after pollination. A phylogenetic analysis involving ITS sequences divided the eight N. suaveolens accessions into three distinct clades. Based on the results, we confirmed that N. suaveolens accessions vary regarding ploidy levels and reproductive isolation mechanisms in crosses with N. tabacum. These accessions will be very useful for revealing and characterizing the reproductive isolation mechanisms in interspecific crosses and their relationships with ploidy levels.

Keywords

Internal transcribed spacer region Interspecific hybridization Phylogenetics Polyploidy Reproductive isolation Tobacco 

Notes

Acknowledgements

This study was partly supported by JSPS KAKENHI Grants (JP20880024 and JP25870627) from the Japan Society for the Promotion of Science.

Supplementary material

10265_2019_1114_MOESM1_ESM.pdf (784 kb)
Supplementary material 1 (PDF 784 kb)

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Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversitySakaiJapan
  2. 2.School of Life and Environmental SciencesOsaka Prefecture UniversitySakaiJapan
  3. 3.Education and Research Field, College of Life, Environment, and Advanced SciencesOsaka Prefecture UniversitySakaiJapan

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