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Enhanced transcriptome responses in herbivore-infested tea plants by the green leaf volatile (Z)-3-hexenol

  • Zhaojun Xin
  • Lingang Ge
  • Shenglong Chen
  • Xiaoling SunEmail author
Regular Paper

Abstract

Green leaf volatiles (GLVs) play a vital role in enhancing herbivore-associated defense responses, but the mechanism by which they precisely regulate such responses is not well understood. (Z)-3-Hexenol (z3HOL), an important component of GLVs, effectively activates the defense of tea plants (Camellia sinensis) against a tea geometrid (TG) Ectropis obliqua Prout. To elucidate the molecular mechanisms of defense activation by z3HOL, RNA-Sequencing was employed to investigate the effect of z3HOL on transcriptome responses to TG in tea plants. A total of 318 upregulated genes were identified, and expression of 10 unigenes was validated by quantitative real-time PCR. Among these 318 upregulated genes, 56 were defense-related, including 6 key enzyme genes in jasmonic acid, and ethylene biosynthesis, 24 signal transduction genes, and 12 insect-responsive transcription factors. Most of the defense-related genes are induced by JA, TG, or wounding treatments, suggesting that JA signaling plays a vital role in z3HOL-induced tea defense against TG.

Keywords

Tea (Z)-3-Hexenol Jasmonic acid RNA-Seq Ectropis obliqua Defense-related genes 

Abbreviations

ACC

1-Aminocyclopropane-1-carboxylic acid

DEGs

Differentially expressed genes

DGE

Digital gene expression

GC–MS

Gas chromatography-mass spectrometry

GLVs

Green leaf volatiles

HIPVs

Herbivore-induced plant volatiles

JA

Jasmonic acid

MPKs

Mitogen-activated protein kinases

PIs

Proteinase inhibitors

PPO

Polyphenol oxidase

qRT-PCR

Quantitative real-time PCR

RH

Relative air humidity

RLKs

Receptor-like protein kinases

RNA-seq

RNA sequencing

TFs

Transcription factors

TG

Tea geometrid

TPS

Terpene synthase

XIPs

Xylanase inhibitor proteins

z3HOL

(Z)-3-Hexenol

Notes

Acknowledgements

The study was jointly sponsored by the Special Fund for Agro-scientific Research in the Public Interest (201403030), the Central Public-interest Scientific Institution Basal Research Fund (no. 1610212016019), the National Natural Science Foundation of China (31401758), and the Science and Technology Program of Zhejiang Province (2016C32026).

Supplementary material

10265_2019_1094_MOESM1_ESM.pdf (220 kb)
Supplementary material 1 (PDF 219 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Zhaojun Xin
    • 1
    • 2
  • Lingang Ge
    • 1
    • 2
  • Shenglong Chen
    • 1
    • 2
  • Xiaoling Sun
    • 1
    • 2
    Email author
  1. 1.Tea Research InstituteChinese Academy of Agricultural SciencesHangzhouChina
  2. 2.Key Laboratory of Tea Biology and Resource Utilization of Ministry of AgricultureHangzhouChina

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