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Journal of Plant Research

, Volume 131, Issue 6, pp 1015–1028 | Cite as

The role of calcium, silicon and salicylic acid treatment in protection of canola plants against boron toxicity stress

  • Ashraf M. Metwally
  • Abeer A. Radi
  • Rasha M. El-Shazoly
  • Afaf M. HamadaEmail author
Regular Paper

Abstract

Boron (B) toxicity often limits crop yield and the quality of production in agricultural areas. Here, we investigated the effects of calcium (Ca), silicon (Si) and salicylic acid (SA) on development of B toxicity, B allocation in canola (Brassica napus cultivar Sarw 4) and its role in non-enzymatic antioxidants in relation to yield of this cultivar under B toxicity. Canola seedlings were subjected to four B levels induced by boric acid in the absence or presence of Ca, Si and SA. The results showed that Ca, Si and SA addition ameliorated the inhibition in canola growth, water content (WC), and improved siliqua number, siliqua weight and seed index. The B content in shoots and roots and total B accumulation in the whole plant were increased in control plants under B-toxicity-stress, and these parameters were significantly decreased by addition of Ca, Si and SA. The shoot ascorbate pool (ascorbate, AsA, and dehydroascorbate, DHA), α-tocopherol and phenolics (free and bound) were increased under B toxicity, and were significantly decreased in most cases by addition of Ca, Si and SA, except α-tocopherol, which increased at low B levels (0, 25 and 50 mg kg soil−1). The glutathione content did not obviously change by B stress, while added Ca, Si and SA inhibited its accumulation under B stress. In addition, B toxicity reduced the shoot flavonoids content; however, this reduction was not alleviated by the use of Ca, Si and SA treatments. It could be concluded that growth and yield of canola plants grown under high B concentration improved after external application of Ca, Si or SA.

Keywords

Boron toxicity Canola (Brassica napus L. cultivar Serw 4) Calcium Salicylic acid Silicon 

Supplementary material

10265_2018_1008_MOESM1_ESM.pdf (3 mb)
Supplementary material 1 (PDF 3067 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Ashraf M. Metwally
    • 1
    • 3
  • Abeer A. Radi
    • 1
  • Rasha M. El-Shazoly
    • 2
  • Afaf M. Hamada
    • 1
  1. 1.Botany and Microbiology Department, Faculty of ScienceAssiut UniversityAssiutEgypt
  2. 2.Botany Department, Faculty of ScienceAssiut UniversityNew ValleyEgypt
  3. 3.Biological Sciences Department, Faculty of ScienceKing Faisal UniversityHofufSaudi Arabia

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