Viridiuvalis adhaerens gen. et sp. nov., a novel colony-forming chlorarachniophyte

Journal of Plant Research volume 130, pages999–1012(2017)Cite this article

Abstract

A new chlorarachniophyte, Viridiuvalis adhaerens gen. et sp. nov. was isolated from the mucus on a coral reef from Zanpa Beach, Okinawa, Japan. The main vegetative stage of V. adhaerens consisted of unicellular coccoid cells with cell walls, although sarcinoid colonies and uniflagellate zoospores were also observed. V. adhaerens had chloroplasts with nucleomorphs and pyrenoids that were completely embedded in the chloroplast. A deep plate-like invagination of the periplastidal compartment (PPC) almost partitioned the pyrenoid and chloroplast components, which were surrounded by two membranes. The nucleomorph was positioned in the base of the invagination of the PPC. Molecular phylogenetic analyses using rRNA genes showed that V. adhaerens branched as a sister lineage of the Amorphochlora clade. The sarcinoid colony, pyrenoid embedded in the chloroplast, and nucleomorph located at the base of the deep invagination of the PPC have not been reported in other chlorarachniophytes. Based on these morphological and ultrastructural characteristics and the results of the molecular phylogenetic analyses, we propose V. adhaerens as a new genus and species of chlorarachniophyte.

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Acknowledgements

This work was supported by JSPS KAKENHI Grant Number 13J00587.

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Affiliations

  1. Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8572, Japan

    Takashi Shiratori, Takeshi Nakayama & Ken-ichiro Ishida

  2. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8572, Japan

    Sakuya Fujita

  3. Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, 565-0871, Japan

    Tenma Shimizu

Authors
  1. Takashi Shiratori
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  2. Sakuya Fujita
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  3. Tenma Shimizu
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  4. Takeshi Nakayama
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  5. Ken-ichiro Ishida
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Correspondence to Takashi Shiratori.

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Takashi Shiratori and Sakuya Fujita have contributed equally to this work.

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Shiratori, T., Fujita, S., Shimizu, T. et al. Viridiuvalis adhaerens gen. et sp. nov., a novel colony-forming chlorarachniophyte. J Plant Res 130, 999–1012 (2017). https://doi.org/10.1007/s10265-017-0961-1

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Keywords

  • Chlorarachniophyceae
  • Chlorarachniophycota
  • Phylogeny
  • Taxonomy
  • Viridiuvalis adhaerens
  • Ultrastructure