WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed
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Callus formation and de novo organogenesis often occur in the wounded tissues of plants. Although this regenerative capacity of plant cells has been utilized for many years, molecular basis for the wound-induced acquisition of regeneration competency is yet to be elucidated. Here we find that wounding treatment is essential for shoot regeneration from roots in the conventional tissue culture of Arabidopsis thaliana. Furthermore, we show that an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) plays a pivotal role for the acquisition of regeneration competency in the culture system. Ectopic expression of WIND1 can bypass both wounding and auxin pre-treatment and increase de novo shoot regeneration from root explants cultured on shoot-regeneration promoting media. In Brassica napus, activation of Arabidopsis WIND1 also greatly enhances de novo shoot regeneration, further corroborating the role of WIND1 in conferring cellular regenerative capacity. Our data also show that sequential activation of WIND1 and an embryonic regulator LEAFY COTYLEDON2 enhances generation of embryonic callus, suggesting that combining WIND1 with other transcription factors promote efficient and organ-specific regeneration. Our findings in the model plant and crop plant point to a possible way to efficiently induce callus formation and regeneration by utilizing transcription factors as a molecular switch.
KeywordsRegeneration Callus formation Plant tissue culture Phytohormones AP2/ERF transcription factor
We thank Mariko Mouri, Chika Ikeda, and Yasuko Yatomi for their technical supports. We are also grateful to David Favero for his linguistic advice. This work was supported by Grants-in-Aid for Scientific Research on Innovative Areas (Grant No. 22119010) and the Research and development projects for application in promoting new policy of agriculture, forestry and fisheries to Sugimoto K. Iwase A was funded by the RIKEN Special Postdoctoral Researchers Program and a grant from Japan Society for the Promotion of Science (Grant No. 24770053). The tomato resources used in this research were provided by the National BioResource Project (NBRP), Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The production of transgenic rapeseed and tomato plants was supported by the RIKEN Plant Transformation Network.