Journal of Plant Research

, Volume 127, Issue 4, pp 525–532 | Cite as

Regulation of jasmonic acid biosynthesis by silicon application during physical injury to Oryza sativa L.

  • Yoon-Ha Kim
  • Abdul Latif Khan
  • Muhammad Waqas
  • Hee-Jeong Jeong
  • Duk-Hwan Kim
  • Jeong Sheop Shin
  • Jong-Guk Kim
  • Myung-Hun Yeon
  • In-Jung Lee
Regular Paper


We investigated the effects of silicon (Si) application on rice plants (Oryza sativa L.) and its responses in the regulation of jasmonic acid (JA) during wounding stress. Endogenous JA was significantly higher in wounded rice plants than in non-wounded. In contrast, Si treatment significantly reduced JA synthesis as compared to non-Si applications under wounding stress. mRNA expression of O. sativa genes showed down-regulation of lipoxygenase, allene oxide synthase 1, allene oxide synthase 2, 12-oxophytodienoate reductase 3, and allene oxide cyclase upon Si application and wounding stress as compared to non-Si-treated wounded rice plants. The physical injury-induced-oxidative stress was modulated by Si treatments, which resulted in higher catalase, peroxidase, and polyphenol oxidase activities as compared with non-Si-treated plants under wounding stress. The higher Si accumulation in rice plants also reduced the level of lipid peroxidation, which helped the rice plants to protect it from wounding stress. In conclusion, Si accumulation in rice plants mitigated the adverse effects of wounding through regulation of antioxidants and JA.


Jasmonic acid biosynthesis Wounding stress Silicon Plant growth Lipid peroxidation 



This research was funded by the Eco-Innovation Project, Korean Governments R&D program on Environmental Technology and Development, Republic of Korea.

Supplementary material

10265_2014_641_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)


  1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–127PubMedCrossRefGoogle Scholar
  2. Agrawal GK, Jwa NS, Agrawal SK, Tamogami S, Iwahashi H, Rakwal R (2003) Cloning of novel rice allene oxide cyclase (OsAOC): mRNA expression and comparative analysis with allene oxide synthase (OsAOS) gene provides insight into the transcriptional regulation of octadecanoid pathway biosynthetic genes in rice. Plant Sci 164:979–992CrossRefGoogle Scholar
  3. Bell E, Mullet JE (1991) Lipoxygenase gene expression is modulated in plants by water deficit, wounding, and methyl jasmonates. Mol Genet 230:456–462CrossRefGoogle Scholar
  4. Bockhaven JV, Vleesschauwer DD, Höfte M (2012) Towards establishing broad-spectrum disease resistance in plants: silicon leads the way. J Exp Bot 64:1281–1293PubMedCrossRefGoogle Scholar
  5. Brady AP, Brown AG, Huff H (1953) The polymerization of aqueous potassium silicate solutions. J Colloid Sci 8:252–276CrossRefGoogle Scholar
  6. Cai K, Gao D, Chen J, Luo S (2009) Probing the mechanisms of silicon-mediated pathogen resistance. Plant Signal Behav 4:1–3PubMedCentralPubMedCrossRefGoogle Scholar
  7. Constabel CP, Ryan CA (1998) A survey of wound- and methyl jasmonate-induced leaf polyphenol oxidase in crop plants. Phytochemistry 47:507–511CrossRefGoogle Scholar
  8. Currie HA, Perry CC (2007) Silica in plants: biological biochemical and chemical studies. Ann Bot 100:1383–1389PubMedCentralPubMedCrossRefGoogle Scholar
  9. Davey MW, Stals E, Panis B, Keulemans J, Swennen RL (2005) High-throughput determination of malondialdehyde in plant tissues. Anal Biochem 347:201–207PubMedCrossRefGoogle Scholar
  10. Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135:1–9CrossRefGoogle Scholar
  11. Dombrowski JE (2003) Salt stress activation of wound-related genes in tomato plants. Plant Physiol 132:2098–2107PubMedCentralPubMedCrossRefGoogle Scholar
  12. Epstein E (2009) Silicon: its manifold roles in plants. Ann Appl Biol 155:155–160CrossRefGoogle Scholar
  13. Eraslan AI, Pilbeam DJ, Gunes A (2008) Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activity in spinach (Spinacia oleracea L. cv. Matador) grown under boron toxicity and salinity. Plant Growth Regul 55:207–219CrossRefGoogle Scholar
  14. Esterbauer H, Schaur RJ, Zollner H (1991) Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde, and related aldehydes. Free Radic Biol Med 11:81–128PubMedCrossRefGoogle Scholar
  15. Farmer EE, Almeras E, Krishnamurthy V (2003) Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory. Curr Opin Plant Biol 6:372–378PubMedCrossRefGoogle Scholar
  16. Garbuzov M, Reidinger S, Susan E (2011) Interactive effects of plant-available soil silicon and herbivory on competition between two grass species. Ann Bot 108:1355–1363PubMedCentralPubMedCrossRefGoogle Scholar
  17. Gfeller A, Baerenfaller K, Loscos J, Che´telat A, Baginsky S, Farmer EE (2011) Jasmonate controls polypeptide patterning in undamaged tissue in wounded Arabidopsis leaves. Plant Physiol 156:1797–1807PubMedCentralPubMedCrossRefGoogle Scholar
  18. Gong H, Zhu X, Chen K, Wang S, Zhang C (2005) Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci 169:313–321CrossRefGoogle Scholar
  19. Hamayun M, Sohn EY, Khan SA, Shinwari ZK, Khan AL, Lee IJ (2010) Silicon alleviates the adverse effects of salinity and drought stress on growth and endogenous plant growth hormones of soybean (Glycine max L.). Pak J Bot 42:1713–1722Google Scholar
  20. Kar M, Mishra D (1976) Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57:315–319PubMedCentralPubMedCrossRefGoogle Scholar
  21. Kim YH, Khan AL, Hamayun M, Kang SM, Beom YJ, Lee IJ (2011) Influence of short-term silicon application on endogenous physiohormonal levels of Oryza sativa L. under wounding stress. Biol Trace Elem Res 144:1175–1185PubMedCrossRefGoogle Scholar
  22. Kim YH, Khan AL, Hamayun M, Kang SM, Lee IJ (2012) Silicon treatment to rice (Oryza sativa L. cv. ‘Gopumbyeo’) plants during different growth periods and its effects on growth and grain yield. Pak J Bot 44:891–897Google Scholar
  23. Kim YH, Khan AL, Waqas M, Shim JK, Kim DH, Lee KY, Lee IJ (2013) Silicon application to rice root zone influenced the phytohormonal and antioxidant responses under salinity stress. J Plant Growth Regul. doi: 10.1007/s00344-013-9356-2 Google Scholar
  24. Kim YH, Khan AL, Kim DH, Lee SY, Kim KM, Waqas M, Jung HY, Shin JH, Kim JG, Lee IJ (2014) Silicon mitigates heavy metal stress by regulating P-type heavy metal ATPases, Oryza sativa low silicon genes, and endogenous phytohormones. BMC Plant Biol 14:13PubMedCentralPubMedCrossRefGoogle Scholar
  25. Kramell R, Atzorn R, Schneider G, Miersch O, Brückner C, Chmidt J, Sembdner G, Parthier B (1995) Occurrence and identification of jasmonic acid and its amino acid conjugates induced by osmotic stress in barley leaf tissue. J Plant Growth Regul 14:29–36CrossRefGoogle Scholar
  26. Lee MW, Qi M, Yang Y (2001) A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death. Mol Plant Microbe Interact 14:527–535PubMedCrossRefGoogle Scholar
  27. Lee A, Cho K, Jang S, Rakwal R, Iwahashi H, Agrawal GK, Shim J, Han O (2004) Inverse correlation between jasmonic acid and salicylic acid during early wound response in rice. Biochem Biophysiol Res Commun 318:734–738CrossRefGoogle Scholar
  28. Leon J, Rojo E, Serrano JJ (2001) Wound signaling in plants. J Exp Bot 52:1–9PubMedCrossRefGoogle Scholar
  29. Li C, Schilmiller AL, Liu GL, Lee GI, Jayanty S, Sageman C (2005) Role of β-oxidation in jasmonate biosynthesis and systemic wound signaling in tomato. Plant Cell 17:971–986PubMedCentralPubMedCrossRefGoogle Scholar
  30. Liang Y, Wong J, Wei L (2005) Silicon-mediated enhancement of cadmium tolerance in maize (Zea mays L.) grown in cadmium contaminated soil. Chemosphere 58:475–483PubMedCrossRefGoogle Scholar
  31. Ma JF, Takahashi E. 2002. Soil, Fertilizer, and Plant Silicon Research in Japan: Netherlands: Elsevier Science B.V. Chapter 6, Silicon uptake and accumulation in plants; p. 73–106Google Scholar
  32. Ma JF, Yamaji N (2008) Functions and transport of silicon in plants. Cell Mol Life Sci 65:3049–3057PubMedCrossRefGoogle Scholar
  33. Ma JF, Yamaji N, Mitani-Ueno N (2011) Transport of silicon from roots to panicles in plants. Jpn Acad Series B 87:377–385CrossRefGoogle Scholar
  34. Massey FP, Hartley SE (2009) Physical defences wear you down: progressive and irreversible impacts of silica on insect herbivores. J Anim Ecol 78:281–291PubMedCrossRefGoogle Scholar
  35. McCloud ES, Baldwin IT (1997) Herbivory and caterpillar regurgitants amplify the wound-induced increases in jasmonic acid but not nicotine in Nicotiana sylvestris. Planta 203:430–435CrossRefGoogle Scholar
  36. Mei CS, Qi M, Sheg GY, Yang YN (2006) Inducible over-expression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection. Mol Plant Microbe Interact 19:1127–1137PubMedCrossRefGoogle Scholar
  37. Mittler R, Vanderauwera S, Suzuki N, Miller G, Tognetti VB, Vandepoele K, Gollery M, Shulaev V, Breusegem FV (2011) ROS signaling: the new wave? Trends Plant Sci 16:300–309PubMedCrossRefGoogle Scholar
  38. Ohkawa H, Ohishi N, Yagi K (1979) Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem 95:351–358PubMedCrossRefGoogle Scholar
  39. Reyes F, Luis CZ (2003) Wounding stress increases the phenolic content and antioxidant capacity of purple-flesh potatoes (Solanum tuberosum L.). Agri Food Chem 51:5296–5300CrossRefGoogle Scholar
  40. Sato C, Aikawa K, Sugiyama S, Nabeta K, Masuta C, Matsuura H (2011) Distal transport of exogenously applied jasmonoyl-isoleucine with wounding stress. Plant Cell Physiol 52:509–517PubMedCrossRefGoogle Scholar
  41. Sattler SE, Meńe-Saffrané L, Farmer EE, Krischke M, Mueller MJ, DellaPennaa D (2006) Nonenzymatic lipid peroxidation reprograms gene expression and activates defense markers in Arabidopsis tocopherol-deficient mutants. Plant Cell 18:3706–3720PubMedCentralPubMedCrossRefGoogle Scholar
  42. Schaller F, Biesgen C, Müssig C, Altmann T, Weiler EW (2000) 12-Oxophytodienoate reductase 3 (OPR3) is the isoenzyme involved in jasmonate biosynthesis. Planta 21:979–984CrossRefGoogle Scholar
  43. Silva MRJ, Pereira SC, Rodrigues FA, Zanão LA Jr, Fontes RLF, Oliveira MGA (2012) Silicon and manganese on the activity of enzymes involved in rice resistance against brown spot. Trop Plant Pathol 37:339–345CrossRefGoogle Scholar
  44. Wasternack C, Stenzel I, Hause B, Hause G, Kutter C, Maucher H, Neumerkel J, Feussner I, Miersch O (2006) The wound response in tomato-role of jasmonic acid. J Plant Physiol 163:297–306PubMedCrossRefGoogle Scholar
  45. Weber H, Chételat A, Reymond P, Farmer EE (2004) Selective and powerful stress gene expression in Arabidopsis in response to malondialdehyde. Plant J 37:877–888PubMedCrossRefGoogle Scholar
  46. Yoshida S, Ohnishi Y, Kitagishi K (1959) Role of silicon in rice nutrition. Soil Plant Food 5:127–133CrossRefGoogle Scholar
  47. Zarate SI, Kempema LA, Walling LL (2007) Silverleaf whitefly induces salicylic acid defenses and suppresses effectual jasmonic acid defenses. Plant Physiol 143:866–875PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan 2014

Authors and Affiliations

  • Yoon-Ha Kim
    • 1
  • Abdul Latif Khan
    • 1
    • 2
  • Muhammad Waqas
    • 1
  • Hee-Jeong Jeong
    • 3
    • 4
  • Duk-Hwan Kim
    • 1
  • Jeong Sheop Shin
    • 3
  • Jong-Guk Kim
    • 5
  • Myung-Hun Yeon
    • 6
  • In-Jung Lee
    • 1
  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Biological Science and ChemistryUniversity of NizwaNizwaOman
  3. 3.School of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea
  4. 4.Department of Plant Molecular Systems Biotechnology and Crop Biotech InstituteKyung Hee UniversityYonginRepublic of Korea
  5. 5.Department of Life Sciences and BiotechnologyKyungpook National UniversityDaeguRepublic of Korea
  6. 6.Nature Conservation Research Division, Environmental Resources DepartmentNational Institute of Environmental ResearchIncheonRepublic of Korea

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