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Journal of Plant Research

, Volume 126, Issue 6, pp 775–786 | Cite as

Boron excess affects photosynthesis and antioxidant apparatus of greenhouse Cucurbita pepo and Cucumis sativus

  • Marco Landi
  • Damiano Remorini
  • Alberto Pardossi
  • Lucia GuidiEmail author
Regular Paper

Abstract

This study aimed to evaluate the behavior of zucchini (Cucurbita pepo L.) and cucumber (Cucumis sativus L.) under boron (B) excess. Plants were grown under greenhouse conditions in a sandy soil–peat mixture using a nutrient solution containing 0.2 (control), 10 and 20 mg L−1 B. Visible symptoms were quantified and leaf B accumulation, gas exchanges, chlorophyll (Chl) a fluorescence, malondialdehyde by-products and antioxidants were investigated 20 days after the beginning of the treatments. Boron toxicity induced oxidative load and leaf necrotic burns coupled with the reduction of leaf growth and biomass accumulation in both species. Boron excess resulted in a decrease of Chl a/b ratio, potential (Fv/Fm) and actual (ΦPSII) PSII quantum efficiency, photosynthetic rate (Pn), stomatal conductance (gs), and transpiration (E) as well. A general stimulation of the antioxidant enzymes ascorbate peroxidase, catalase and superoxide dismutase was observed, and a significant increase in the oxidized form of ascorbate and glutathione was evidenced for treated plants of both species. A difference between the two species was observed: C. pepo appeared to be more sensitive to B stress being damaged at all B concentration. C. sativus grown at 10 mg L−1 B in nutrient solution showed some down-regulated mechanisms, i.e. increase in Chl b content and a good photochemical PSII efficiency as well as a higher amount of constitutive antioxidant molecules, that, however, are not sufficient to contrast the negative effects of B.

Keywords

Boron toxicity Chlorophyll a fluorescence Cucumber Gas exchanges Photosynthesis Zucchini 

Notes

Acknowledgments

This work was funded by MIUR-PRIN 2009 (Ministero dell’Istruzione, dell’Università e della Ricerca, Italy, Project “Physiological response of vegetables crops to boron excess”).

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Copyright information

© The Botanical Society of Japan and Springer Japan 2013

Authors and Affiliations

  • Marco Landi
    • 1
  • Damiano Remorini
    • 1
  • Alberto Pardossi
    • 1
  • Lucia Guidi
    • 1
    Email author
  1. 1.Department of Agriculture, Food and EnvironmentUniversity of PisaPisaItaly

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