Chitosan and a fungal elicitor inhibit tracheary element differentiation and promote accumulation of stress lignin-like substance in Zinnia elegans xylogenic culture
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We investigated the effect of elicitors on xylem differentiation and lignification using a Zinnia elegans xylogenic culture system. Water-soluble chitosan and a fungal elicitor derived from Botrytis cinerea were used as elicitors. Elicitor addition at the start of culturing inhibited tracheary element (TE) differentiation in a concentration-dependent manner, and 30 μg mL−1 of chitosan or 16.7 μg mL−1 of the fungal elicitor strikingly inhibited TE differentiation and lignification. Addition of chitosan (at 50 μg mL−1) or the fungal elicitor (at 16.7 μg mL−1) during the culturing period also inhibited TE differentiation without inhibiting cell division, except for immature TEs undergoing secondary wall thickening. Elicitor addition after immature TE appearance also caused the accumulation of an extracellular lignin-like substance. It appears that elicitor addition at the start of culturing inhibits the process by which dedifferentiated cells differentiate into xylem cell precursors. Elicitor addition during culturing also appears to inhibit the transition from xylem cell precursors to immature TEs, and induces xylem cell precursors or xylem parenchyma cells to produce an extracellular stress lignin-like substance.
KeywordsBotrytis cinerea Chitosan Fungal elicitor Stress lignin Tracheary element differentiation Zinnia elegans
Microbe-associated molecular pattern
This work was supported in part by project grants to Y. S from Ehime University, Japan (Rudimentary Research Support) and from the Faculty of Science, Ehime University (Research Support).
- Hano C, Addi M, Bensaddek L, Cronier D, Baltora-Rosset S, Doussot J, Maury S, Mesnard F, Chabbert B, Hawkins S, Laine E, Lamblin F (2006) Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures. Planta 223:975–989PubMedCrossRefGoogle Scholar
- Lange BM, Lapierre C, Sandermann H Jr (1995) Elicitor-induced spruce stress lignin (structural similarity to early developmental lignins). Plant Physol 108:1277–1287Google Scholar
- Naoumkina MA, Zhao Q, Gallego-Giraldo L, Dai X, Zhao PX, Dixon RA (2010) Genome-wide analysis of phenylpropanoid defence pathways. Mol Plant Pathol 11:829–846Google Scholar
- Rasband WS (1997–2013) ImageJ, U. S. National Institutes of Health, Bethesda, Maryland. http://rsb.info.nih.gov/ij/
- Sato Y, Demura T, Yamawaki K, Inoue Y, Sato S, Sugiyama M, Fukuda H (2006) Isolation and characterization of a novel peroxidase gene ZPO-C whose expression and function are closely associated with lignification during tracheary element differentiation. Plant Cell Physiol 47:493–503PubMedCrossRefGoogle Scholar
- Suzuki K, Fukuda Y, Shinsh H (1995) Studies on elicitor signal transduction leading to differential expression of defense genes in cultured tobacco cells. Plant Cell Physiol 36:281–289Google Scholar
- Yamaguchi T, Yamada A, Hong N, Ogawa T, Ishii T, Shibuya N (2000) Differences in the recognition of glucan elicitor signals between rice and soybean: β-glucan fragments from the rice blast disease fungus Pyricularia oryzae that elicit phytoalexin biosynthesis in suspension-cultured rice cells. Plant Cell 12:817–826PubMedGoogle Scholar