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Journal of Plant Research

, Volume 126, Issue 3, pp 415–425 | Cite as

Sugar beet M14 glyoxalase I gene can enhance plant tolerance to abiotic stresses

  • Chuan Wu
  • Chunquan Ma
  • Yu Pan
  • Shilong Gong
  • Chenxi Zhao
  • Sixue Chen
  • Haiying Li
Regular Paper

Abstract

Glyoxalase I is the first enzyme of the glyoxalase system that can detoxify methylglyoxal, a cytotoxic compound increased rapidly under stress conditions. Here we report cloning and characterization of a glyoxalase I from sugar beet M14 line (an interspecific hybrid between a wild species Beta corolliflora Zoss and a cultivated species B. vulgaris L). The full-length gene BvM14-glyoxalase I has 1,449 bp in length with an open reading frame of 1,065 bp encoding 354 amino acids. Sequence analysis shows the conserved glyoxalase I domains, metal and glutathione binding sites and secondary structure (α-helixes and β-sheets). The BvM14-glyoxalase I gene was ubiquitously expressed in different tissues of sugar beet M14 line and up-regulated in response to salt, mannitol and oxidative stresses. Heterologous expression of BvM14-glyoxalase I could increase E. coli tolerance to methylglyoxal. Transgenic tobacco plants constitutively expressing BvM14-glyoxalase I were generated. Both leaf discs and seedlings showed significant tolerance to methylglyoxal, salt, mannitol and H2O2. These results suggest an important role of BvM14-glyoxalase I in cellular detoxification and tolerance to abiotic stresses.

Keywords

Sugar beet M14 BvM14-glyoxalase I Methylglyoxal detoxification Stress tolerance 

Notes

Acknowledgments

This research was supported by the National Science Foundation of China (Project 31071473: Studies on the function of the BvM14-cystatin in sugar beet M14 lines, and Project 30871566: Studies on floral organ-specific expressed proteins in sugar beet M14 lines), and the Graduate Innovation Project of Heilongjiang Province. The paper represents serial 006 from our innovation team at the Heilongjiang University (Hdtd2010-05).

Supplementary material

10265_2012_532_MOESM1_ESM.pdf (85 kb)
Supplementary material 1 (PDF 85 kb)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2012

Authors and Affiliations

  • Chuan Wu
    • 1
  • Chunquan Ma
    • 1
  • Yu Pan
    • 1
  • Shilong Gong
    • 1
  • Chenxi Zhao
    • 1
  • Sixue Chen
    • 1
    • 2
  • Haiying Li
    • 1
    • 3
  1. 1.Key Laboratory of Molecular Biology, College of Heilongjiang Province, College of Life SciencesHeilongjiang UniversityHarbinChina
  2. 2.Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology ProgramUniversity of FloridaGainesvilleUSA
  3. 3.Engineering Research Center of Agricultural Microbiology TechnologyMinistry of Education Heilongjiang UniversityHarbinChina

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