Sugar beet M14 glyoxalase I gene can enhance plant tolerance to abiotic stresses
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Glyoxalase I is the first enzyme of the glyoxalase system that can detoxify methylglyoxal, a cytotoxic compound increased rapidly under stress conditions. Here we report cloning and characterization of a glyoxalase I from sugar beet M14 line (an interspecific hybrid between a wild species Beta corolliflora Zoss and a cultivated species B. vulgaris L). The full-length gene BvM14-glyoxalase I has 1,449 bp in length with an open reading frame of 1,065 bp encoding 354 amino acids. Sequence analysis shows the conserved glyoxalase I domains, metal and glutathione binding sites and secondary structure (α-helixes and β-sheets). The BvM14-glyoxalase I gene was ubiquitously expressed in different tissues of sugar beet M14 line and up-regulated in response to salt, mannitol and oxidative stresses. Heterologous expression of BvM14-glyoxalase I could increase E. coli tolerance to methylglyoxal. Transgenic tobacco plants constitutively expressing BvM14-glyoxalase I were generated. Both leaf discs and seedlings showed significant tolerance to methylglyoxal, salt, mannitol and H2O2. These results suggest an important role of BvM14-glyoxalase I in cellular detoxification and tolerance to abiotic stresses.
KeywordsSugar beet M14 BvM14-glyoxalase I Methylglyoxal detoxification Stress tolerance
This research was supported by the National Science Foundation of China (Project 31071473: Studies on the function of the BvM14-cystatin in sugar beet M14 lines, and Project 30871566: Studies on floral organ-specific expressed proteins in sugar beet M14 lines), and the Graduate Innovation Project of Heilongjiang Province. The paper represents serial 006 from our innovation team at the Heilongjiang University (Hdtd2010-05).
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