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Journal of Plant Research

, Volume 126, Issue 2, pp 243–250 | Cite as

Acquisition of embryogenic competency does not require cell division in carrot somatic cell

  • Akira KikuchiEmail author
  • Masashi Asahina
  • Motoki Tanaka
  • Shinobu Satoh
  • Hiroshi Kamada
Regular Paper

Abstract

Totipotency is the ability of a cell to regenerate the entire organism, even after previous differentiation as a specific cell. When totipotency is coupled with active cell division, it was presumed that cell division is essential for this expression. Here, using the stress-induction system of somatic embryos in carrots, we show that cell division is not essential for the expression of totipotency in somatic/embryonic conversion. Morphological and histochemical analyses showed that the cell did not divide during embryo induction. Inhibitors of cell division did not affect the rate of somatic embryo formation. Our results indicate that the newly acquired trait of differentiation appears without cell division, but does not arise with cell division as a newborn cell.

Keywords

Carrot (Daucus carotaCell division Somatic embryogenesis Totipotency 

Abbreviations

AP

Aphidicolin

BrdU

5-Bromo-2-deoxyuridine

DAIP

4′,6-Diamidino-2-phenylindole

DMSO

Dimethyl sulfoxide

FdU

5-Fluoro-2′-deoxyuridine

PP

Propyzamide

Notes

Acknowledgments

This work was supported in part by the Grants-in-Aid program from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant no. 23570045) and by the joint research program “Plant Transgenic Research Design, University of Tsukuba.”

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Copyright information

© The Botanical Society of Japan and Springer 2012

Authors and Affiliations

  • Akira Kikuchi
    • 1
    Email author
  • Masashi Asahina
    • 1
    • 2
  • Motoki Tanaka
    • 1
    • 3
  • Shinobu Satoh
    • 1
  • Hiroshi Kamada
    • 1
  1. 1.Faculty of Life and Environmental Sciences, Gene Research CenterUniversity of TsukubaTsukubaJapan
  2. 2.Department of BiosciencesTeikyo UniversityUtsunomiyaJapan
  3. 3.SDS Biotech K.K., Tsukuba Research and Technology CenterTsukubaJapan

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