Journal of Plant Research

, Volume 125, Issue 2, pp 311–316 | Cite as

Use of intercellular washing fluid to investigate the secreted proteome of the rice–Magnaporthe interaction

  • Matthew R. Shenton
  • Thomas Berberich
  • Masaharu Kamo
  • Tetsuro Yamashita
  • Hideharu Taira
  • Ryohei Terauchi
Technical Note

Abstract

Early interactions between invading penetration hyphae of the pathogenic fungus Magnaporthe oryzae and rice cells occur at the apoplast, the free diffusional space outside the plasma membrane of leaves. After initial colonization, intercellular hyphae are again in intimate contact with the rice apoplast. While several studies have looked at proteomics in rice–Magnaporthe interactions, none have focused on apoplast localized proteins. We adjusted a protocol for intercellular washing fluids (IWF) to rice leaves infected with Magnaporthe oryzae for proteomic analysis. In our IWF extract, we identified several proteins associated with compatible or incompatible pathogen interactions. Three DUF26 domain proteins were identified as changing in abundance 12 h after inoculation, confirming DUF26 domain-containing proteins are among early, pathogen stress-responsive proteins induced by infection with Magnaporthe oryzae. A Magnaporthe cyclophilin, previously identified as a virulence factor was also identified in the intercellular washing fluid.

Keywords

2D-PAGE Magnaporthe Protein extraction Rice Secreted proteins 

Notes

Acknowledgments

This work was supported by ‘Program for Promotion of Basic Research Activities for Innovative Bioscience’ to RT. TB is supported by the research funding programme “LOEWE—Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz” of Hesse’s Ministry of Higher Education, Research, and the Arts.

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Copyright information

© The Botanical Society of Japan and Springer 2012

Authors and Affiliations

  • Matthew R. Shenton
    • 1
    • 4
  • Thomas Berberich
    • 1
    • 5
  • Masaharu Kamo
    • 2
  • Tetsuro Yamashita
    • 3
  • Hideharu Taira
    • 3
  • Ryohei Terauchi
    • 1
  1. 1.Iwate Biotechnology Research CenterKitakamiJapan
  2. 2.Iwate Medical UniversityMoriokaJapan
  3. 3.Iwate UniversityMoriokaJapan
  4. 4.National Institute of GeneticsMishimaJapan
  5. 5.Biodiversity and Climate Research Centre (BiK-F)Frankfurt/MainGermany

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