Journal of Plant Research

, Volume 125, Issue 3, pp 439–449

Degradation of long-chain base 1-phosphate (LCBP) in Arabidopsis: functional characterization of LCBP phosphatase involved in the dehydration stress response

  • Noriko Nakagawa
  • Mai Kato
  • Yohei Takahashi
  • Ken-ichiro Shimazaki
  • Kentarao Tamura
  • Yoshihiko Tokuji
  • Akio Kihara
  • Hiroyuki Imai
Regular Paper


Sphingolipid metabolites, long-chain base 1-phosphates (LCBPs), are involved in ABA signaling pathways. The LCBPs synthesized by long-chain base kinase are dephosphorylated by LCBP phosphatase or degraded by LCBP lyase. Here we show that the At3g58490 gene encodes AtSPP1, a functional LCBP phosphatase. Transient expression of green fluorescent protein fusion in suspension-cultured Arabidopsis cells showed that AtSPP1 is localized in the endoplasmic reticulum. The level of dihydrosphingosine 1-phosphate was increased in loss-of-function mutants (spp1) compared with wild-type (WT) plants, suggesting a role of AtSPP1 in regulating LCBP levels. The rate of decrease in fresh weight of detached aerial parts was significantly slower in spp1 mutants than in WT plants. A stomatal closure bioassay showed that the stomata of spp1 mutants were more sensitive than the WT to ABA, suggesting that AtSPP1 is involved in guard cell signaling. However, spp1 mutants showed decreased sensitivity to ABA with respect to primary root growth but not to seed germination. The response to fumonisin B1 did not differ between the WT and spp1 mutant. A significant decrease in AtDPL1 (LCBP lyase) transcripts in spp1 mutants was observed. We conclude that AtSPP1 is a functional LCBP phosphatase that may play a role in stomatal responses through LCBP-mediated ABA signaling.


ABA Arabidopsis thaliana Dehydration stress Long-chain base 1-phosphate phosphatase Stomatal closure Sphingolipids 





Dihydrosphingosine 1-phosphate


Fumonisin B1


Long-chain base


Long-chain base 1-phosphate




Phytosphingosine 1-phosphate




Sphingosine 1-phosphate

Supplementary material

10265_2011_451_MOESM1_ESM.pdf (123 kb)
Supplementary Fig. S1 (PDF 122 kb)
10265_2011_451_MOESM2_ESM.pdf (132 kb)
Supplementary Fig. S2 (PDF 131 kb)
10265_2011_451_MOESM3_ESM.pdf (98 kb)
Supplementary Fig. S3 (PDF 98 kb)
10265_2011_451_MOESM4_ESM.pdf (60 kb)
Supplementary Table S1 (PDF 60 kb)
10265_2011_451_MOESM5_ESM.pdf (89 kb)
Supplementary Table S2 (PDF 88 kb)

Copyright information

© The Botanical Society of Japan and Springer 2011

Authors and Affiliations

  • Noriko Nakagawa
    • 1
  • Mai Kato
    • 1
  • Yohei Takahashi
    • 2
  • Ken-ichiro Shimazaki
    • 2
  • Kentarao Tamura
    • 3
  • Yoshihiko Tokuji
    • 4
  • Akio Kihara
    • 5
  • Hiroyuki Imai
    • 1
  1. 1.Department of Biology, Graduate School of Natural ScienceKonan UniversityKobeJapan
  2. 2.Department of Biology, Faculty of ScienceKyushu UniversityFukuokaJapan
  3. 3.Department of Botany, Graduate School of ScienceKyoto UniversityKyotoJapan
  4. 4.Department of Food ScienceObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  5. 5.Laboratory of Biochemistry, Faculty of Pharmaceutical SciencesHokkaido UniversitySapporoJapan

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