Advertisement

Journal of Plant Research

, Volume 125, Issue 3, pp 429–438 | Cite as

The Arabidopsis AtbZIP1 transcription factor is a positive regulator of plant tolerance to salt, osmotic and drought stresses

  • Xiaoli Sun
  • Yong Li
  • Hua Cai
  • Xi Bai
  • Wei Ji
  • Xiaodong DingEmail author
  • Yanming ZhuEmail author
Regular Paper

Abstract

According to the AtGenExpress transcriptome data sets, AtbZIP1 is an Arabidopsis gene induced by several abiotic stresses, such as salt, cold and drought. Here, we isolated AtbZIP1, and used semi-quantitative reverse transcription-PCR to verify that AtbZIP1 expression was indeed significantly induced by salt, osmotic, and cold stresses in Arabidopsis. AtbZIP1 knockout mutants showed a reduced tolerance to salt and osmotic stresses, coinciding with a suppression of the expression of several stress-responsive genes, such as COR15A, RD17 and RD29A. Consistently, the restoration of AtbZIP1 in the knockout lines restored the plants ability to tolerate salt and osmotic stresses. Furthermore, overexpressing AtbZIP1 in the wild type Arabidopsis resulted in an enhanced tolerance to salt and drought stresses. Sequence analysis shows that AtbZIP1 belongs to the S subfamily of basic leucine zipper transcription factors (TFs). The transient expression of green fluorescent protein-AtbZIP1 in tobacco leaf cells showed that AtbZIP1 localizes in nuclei. A transactivation assay further suggested that AtbZIP1 functions as a transcriptional activator in yeast and the two protein motifs (aa 13–38 and 92–118) are indispensable for transactivation activity. These results indicate that the TF AtbZIP1 is a positive regulator of plant tolerance to salt, osmotic, and drought stresses.

Keywords

Arabidopsis AtbZIP1 Drought stress Osmotic stress Salt stress 

Notes

Acknowledgments

We would like to thank Drs. Wenyuan Song, Yoshichika Kitagawa and Meghan Hennis for the critical reading of the manuscript and invaluable comments on the work, and NASC for the seeds of SALK_069498C and SALK_059343. This project was supported by the National Natural Science Foundation of China (30570990), the National Major Project for Cultivation of Transgenic Crops (20082x08004), the Key Research Plan of Heilongjiang Province (GA06B103), and the Innovation Research Group of NEAU.

Supplementary material

10265_2011_448_MOESM1_ESM.tif (635 kb)
Supplemental Figure 1 Sequence analysis of the AtbZIP1 gene. a A schematic showing the construction of AtbZIP1, including three subdomains: upstream open-reading-frames (uORF), basic region (BR) and leucine zipper (LZ). Sequence of the upstream open-reading-frames (uORF) of AtbZIP1 was shown. b The alignment of AtbZIP1 and other proteins of S subfamily using CLUSTAL X. ‘‘*’’ indicates positions which have a single, fully conserved residue; ‘‘:’’ indicates highly conserved positions, and dashes (---) indicate gaps in the amino acid sequences. The column show the score of the conservation positions, and the line marked shows the basic region (BR) and leucine zipper (LZ) (TIFF 634 kb)
10265_2011_448_MOESM2_ESM.doc (28 kb)
Supplementary Table S1 (DOC 28 kb)

References

  1. Alonso R, Onate-Sanchez L, Weltmeier F, Ehlert A, Diaz I, Dietrich K, Vicente-Carbajosa J, Droge-Laser W (2009) A pivotal role of the basic leucine zipper transcription factor bZIP53 in the regulation of Arabidopsis seed maturation gene expression based on heterodimerization and protein complex formation. Plant Cell 21:1747–1761PubMedCrossRefGoogle Scholar
  2. Baena-González E, Rolland F, Thevelein JM, Sheen J (2007) A central integrator of transcription networks in plant stress and energy signaling. Nature 448:938–943PubMedCrossRefGoogle Scholar
  3. Beckett D (2001) Regulated assembly of transcription factors and control of transcription initiation. J Mol Biol 314:335–352PubMedCrossRefGoogle Scholar
  4. Choi H, Hong J, Ha J, Kang J, Kim SY (2000) ABFs: a family of ABA-responsive element binding factors. J Biol Chem 275:1723–1730PubMedCrossRefGoogle Scholar
  5. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743PubMedCrossRefGoogle Scholar
  6. Ehlert A, Weltmeier F, Wang X, Mayer CS, Smeekens S, Vicente-Carbajosa J, Droge-Laser W (2006) Two-hybrid protein–protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group-C and S bZIP transcription factors. Plant J 46:890–900PubMedCrossRefGoogle Scholar
  7. Ho LW, Yang TT, Shieh SS, Edwards GE, Yen HE (2010) Reduced expression of a vesicle trafficking-related ATPase SKD1 decreases salt tolerance in Arabidopsis. Funct Plant Biol 37:962–973CrossRefGoogle Scholar
  8. Jakoby M, Dröge-Laser W, Vicente-Carbajosa J (2002) bZIP transcription factors in Arabidopsis. Trends Plant Sci 15:106–111CrossRefGoogle Scholar
  9. Kaminaka H, Nake C, Epple P, Dittgen J, Schutze K, Chaban C, Holt BF III, Merkle T, Schafer E, Harter K, Dangl JL (2006) bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection. EMBO J 25:4400–4411PubMedCrossRefGoogle Scholar
  10. Kang JJ, Choi HH, Im MM, Kim SY (2002) Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14:343–357PubMedCrossRefGoogle Scholar
  11. Kang SG, Price J, Lin PC, Hong JC, Jang JC (2010) The Arabidopsis bZIP1 transcription factor is involved in sugar signaling, protein networking, and DNA binding. Mol Plant 3:361–373PubMedCrossRefGoogle Scholar
  12. Kapila J, De Rycke R, Van Montagu M, Angenon G (1997) An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci 122:101–108CrossRefGoogle Scholar
  13. Kilian J, Whitehead D, Horak J, Wanke D, Weinl S, Batistic O, D’Angelo C, Bornberg-Bauer E, Kudla J, Harter K (2007) The AtGenExpress global stress expression data set: protocols, evaluation and model data analysis of UV-B light, drought and cold stress responses. Plant J 50:347–363PubMedCrossRefGoogle Scholar
  14. Kim SY, Ma J, Perret P, Li Z, Thomas TL (2002) Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities. Plant Physiol 30:688–697CrossRefGoogle Scholar
  15. Lee SC, Choi HW, Hwang IS, Choi DS, Hwang BK (2006) Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIP1, in enhanced resistance to pathogen infection and environmental stresses. Planta 224:1209–1225PubMedCrossRefGoogle Scholar
  16. Liao Y, Zhang JS, Chen SY, Zhang WK (2008) Role of soybean GmbZIP132 under abscisic acid and salt stresses. J Integr Plant Biol 50:221–230PubMedCrossRefGoogle Scholar
  17. Liu JX, Srivastava R, Che P, Howell SH (2007) An endoplasmic reticulum stress response in Arabidopsis is mediated by proteolytic processing and nuclear relocation of a membrane-associated transcription factor, bZIP28. Plant Cell 19:4111–4119PubMedCrossRefGoogle Scholar
  18. Lu GJ, Gao CX, Zheng XN, Han B (2009) Identification of OsbZIP72 as a positive regulator of ABA response and drought tolerance in rice. Planta 229:605–615PubMedCrossRefGoogle Scholar
  19. Satoh R, Fujita Y, Nakashima K, Shinozaki K, Yamaguchi-Shinozaki K (2004) A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis. Plant Physiol 45:309–317Google Scholar
  20. Schutze K, Harter K, Chaban C (2008) Post-translational regulation of plant bZIP factors. Trends Plant Sci 13(5):247–255PubMedCrossRefGoogle Scholar
  21. Shen H, Cao K, Wang X (2007a) A conserved proline residue in the leucine zipper region of AtbZIP34 and AtbZIP61 in Arabidopsis thaliana interferes with the formation of homodimer. BBRC 362:425–430PubMedGoogle Scholar
  22. Shen HS, Cao KM, Wang XP (2007b) AtbZIP16 and AtbZIP68, two new members of GBFs, can interact with other G group bZIPs in Arabidopsis thaliana. BMB Rep 41:132–138CrossRefGoogle Scholar
  23. Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. PNAS 97:11632–11637PubMedCrossRefGoogle Scholar
  24. Walter M, Chaban C, Schutze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J (2004) Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J 40:428–438PubMedCrossRefGoogle Scholar
  25. Weltmeier F, Ehlert A, Mayer CS, Dietrich K, Wang X, Schutze K, Alonso R, Harter K, Vicente-Carbajosa J, Droge-Laser W (2006) Combinatorial control of Arabidopsis proline dehydrogenase transcription by specific heterodimerisation of bZIP transcription factors. EMBO J 25:3133–3143PubMedCrossRefGoogle Scholar
  26. Weltmeier F, Rahmani F, Ehlert A, Dietrich K, Wang X, Schutze K, Chaban C, Hanson J, Teige M, Harter K, Vicente-Carbajosa J, Smeekens S, Droge-Laser W (2009) Expression patterns within the Arabidopsis C/S1 bZIP transcription factor network: availability of heterodimerization partners controls gene expression during stress response and development. Plant Mol Biol 69:107–119PubMedCrossRefGoogle Scholar
  27. Wingender E, Chen X, Fricke E, Geffers R, Hehl R (2001) The TRANSFAC system on gene expression regulation. Nucleic Acids Res 29:281–283PubMedCrossRefGoogle Scholar
  28. Xiang Y, Tang N, Du H, Ye HY, Xiong LZ (2008) Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice. Plant Physiol 148:1938–1952PubMedCrossRefGoogle Scholar
  29. Zou MJ, Guan YC, Ren HB, Zhang F, Chen F (2007) Characterization of alternative splicing products of bZIP transcription factors OsABI5. BBRC 360:307–313PubMedGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer 2011

Authors and Affiliations

  1. 1.Plant Bioengineering LaboratoryNortheast Agricultural UniversityHarbinChina
  2. 2.Department of NeurologyThe University of Texas Southwestern Medical CenterDallasUSA

Personalised recommendations