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Journal of Plant Research

, Volume 125, Issue 1, pp 165–172 | Cite as

Protein dynamics during seed germination under copper stress in Arabidopsis over-expressing Potentilla superoxide dismutase

  • Tejpal Gill
  • Vivek Dogra
  • Sanjay Kumar
  • Paramvir Singh Ahuja
  • Yelam Sreenivasulu
Regular Paper

Abstract

Copper (Cu), though an essential micronutrient for plants, poses toxicity at higher concentrations possibly by inducing oxidative stress. With the background that enzyme superoxide dismutase (SOD) ameliorates oxidative stress, the present work focused on understanding physiological and proteomic response of Arabidopsis seeds constitutively over-expressing copperzinc SOD of Potentilla atrosanguinea (PaSOD) during germination in response to varied concentrations of copper sulphate (Cu stress). Transgenics showed higher germination percentage and required less “mean time to germination” under Cu-stress. In response to Cu stress, 39 differentially expressed protein spots were detected by 2-D electrophoresis in proteins of germinating wild type (WT) and transgenic seeds, of which 14 spots appeared exclusively in transgenics. Among the rest 25 protein spots, 14 showed down-regulation, one showed up-regulation, and 10 spots disappeared. MALDI-TOF and subsequent peptide mass fingerprinting analysis revealed that the down-regulated proteins in transgenics were related to oxidative stress, detoxification, germination, intermediary metabolism and regulatory proteins. Up-regulated proteins in WT and down-regulated proteins in transgenic during Cu stress were the same. Changes in key proteins, vis-à-vis alleviation of oxidative stress in transgenic Arabidopsis over-expressing PaSOD possibly alleviated toxicity of Cu-induced stress during seed germination, resulting in higher germination rate and germination percentage.

Keywords

Arabidopsis thaliana Heavy metal toxicity Oxidative stress Seed germination proteins Transgenics SOD 

Notes

Acknowledgments

Authors thank the Department of Biotechnology, New Delhi, India for financial assistance through the project entitled “Bioprospecting Himalayan Bioresources Through Transgenic and Nutraceutical Technology; BT/PR8876/NDB/52/68/2007”. TG acknowledges receipt of Senior Research Fellowship by the Council of Scientific and Industrial Research (CSIR), New Delhi. The authors are grateful to the Council of Scientific and Industrial Research (CSIR), New Delhi for providing infrastructural support.

Supplementary material

10265_2011_421_MOESM1_ESM.doc (68 kb)
Supplementary table S1 (DOC 67.5 kb)

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Copyright information

© The Botanical Society of Japan and Springer 2011

Authors and Affiliations

  • Tejpal Gill
    • 1
  • Vivek Dogra
    • 1
  • Sanjay Kumar
    • 1
  • Paramvir Singh Ahuja
    • 1
  • Yelam Sreenivasulu
    • 1
  1. 1.Biotechnology DivisionInstitute of Himalayan Bioresource Technology (Council of Scientific and Industrial Research)PalampurIndia

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