Journal of Plant Research

, Volume 125, Issue 1, pp 155–164

Drought-induced proline accumulation is uninvolved with increased nitric oxide, which alleviates drought stress by decreasing transpiration in rice

Regular Paper


Accumulation of proline is trusted to be an adaptive response of plants against drought stress, and exogenous application of nitric oxide (NO) enhances proline accumulation in Cu-treated algae. In order to investigate whether NO works as a necessary signaling molecule in drought-induced proline accumulation in rice leaves, effects of drought stress on endogenous NO content and proline accumulation were studied in rice leaves, using sodium nitroprusside (SNP, a NO donor) and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, a NO scavenger). The results showed that drought treatment increased both endogenous NO and proline contents in rice leaves, while foliar spray of various concentrations of SNP failed to induce proline accumulation in the leaves of well-watered rice and foliar spray of cPTIO failed to inhibit proline accumulation in the leaves of drought-stressed rice. These results indicate that increase of endogenous NO is dispensable for proline accumulation in the leaves of rice under drought stress. Further studies indicate that exogenous application of NO alleviates drought-induced water loss and ion leakage by decreasing transpiration rate of rice leaves.


Abscisic acid Oryza Sodium nitroprusside Transpiration rate Water stress 



Abscisic acid


Cation exchange capacity




4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate


Dry weight


Fresh weight


Nitric oxide


Δ1-Pyrroline-5-carboxylate synthetase


Photosynthetic photo flux density


Sodium nitroprusside


Saturated weight


Reactive oxygen species


Relative water content


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Copyright information

© The Botanical Society of Japan and Springer 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Rice BiologyChina National Rice Research InstituteHangzhouPeople’s Republic of China
  2. 2.State Key Laboratory of Plant Physiology and Biochemistry, College of Life SciencesZhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.College of Life SciencesChina Jiliang UniversityHangzhouPeople’s Republic of China

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