Journal of Plant Research

, Volume 124, Issue 1, pp 49–62 | Cite as

Taxonomy of Nephroselmis viridis sp. nov. (Nephroselmidophyceae, Chlorophyta), a sister marine species to freshwater N. olivacea

  • Haruyo Yamaguchi
  • Shoichiro Suda
  • Takeshi Nakayama
  • Richard N. Pienaar
  • Mitsuo Chihara
  • Isao Inouye
Regular Paper

Abstract

The genus Nephroselmis (Nephroselmidophyceae), which had been placed in the Prasinophyceae, is one of the primitive green flagellates that are important to our understanding of the early evolution of green plants. We studied a new species of Nephroselmis isolated from Japan, Fiji and South Africa. This species has been known for a long time as undescribed species ‘N. viridis.N. viridis possesses some ultrastructural characters shared with only the freshwater type species N. olivacea, including a disc-like structure beneath the pyrenoid and bipolar spiny body scales with 1-5-8-5-1 spines. Molecular phylogenetic analysis based on 18S rDNA also supports a sister relationship between N. viridis and N. olivacea. However, N. viridis is distinguishable from N. olivacea by the shape of its starch sheath, its scales, its pigment composition and its habitat. In this paper, we designate the formal description of N. viridis sp. nov. We also describe variability in the 18S rDNA introns of various N. viridis strains. This detailed study of N. viridis provides some insights into the evolution of Nephroselmis.

Keywords

18S rDNA Intron Nephroselmis viridis Phylogeny Prasinophyceae Ultrastructure 

Supplementary material

10265_2010_349_MOESM1_ESM.pdf (867 kb)
Supplementary material 1 (PDF 867 kb)

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Copyright information

© The Botanical Society of Japan and Springer 2010

Authors and Affiliations

  • Haruyo Yamaguchi
    • 1
  • Shoichiro Suda
    • 2
  • Takeshi Nakayama
    • 1
  • Richard N. Pienaar
    • 3
  • Mitsuo Chihara
    • 1
  • Isao Inouye
    • 1
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Department of Chemistry, Biology and Marine ScienceUniversity of the RyukyusNishiharaJapan
  3. 3.School of Animal, Plant and Environmental SciencesUniversity of the WitwatersrandJohannesburg, WITS 2050South Africa

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