Journal of Plant Research

, Volume 124, Issue 1, pp 155–163 | Cite as

Two CLE genes are induced by phosphate in roots of Lotus japonicus

  • Sachiko Funayama-NoguchiEmail author
  • Ko Noguchi
  • Chie Yoshida
  • Masayoshi Kawaguchi
Regular Paper


Genes of CLE (CLAVATA3/ESR-related) family encode peptide ligands that regulate plant development in response to external stimuli such as rhizobial infection and the nitrate application as well as various internal stimuli. To investigate whether LjCLE gene(s) may involve in plant response to inorganic phosphate (Pi), we analyzed Pi responses of 39 LjCLE genes in hydroponically grown Lotus japonicus plants (ecotype Miyakojima ‘MG-20’). Two LjCLE genes, LjCLE19 and 20, were up-regulated specifically and greatly in roots of L. japonicus by Pi addition to the hydroponic solution. When the external Pi level increased, expressions of LjCLE19 and 20 increased before the increase in the Pi content in plants. On the other hand, when the external Pi level decreased, the Pi content in plants decreased first, then expression levels of LjCLE19 and 20 decreased. Based on our results, we discuss the relationship between LjCLE19 and 20 and the tissue Pi levels in plants. This is the first report showing induction of specific CLE genes by phosphate.


CLE genes Lotus japonicus Phosphate Root 





Fresh weight




Inorganic phosphate



We are grateful to Dr. M. Taira for the use of real-time PCR instrument, to Prof. T. Mimura for his advice on the measurement of Pi, to Dr. S. Yano for his advice on the measurement of root length and to Dr. S. Okamoto and Ms. E. Ohnishi for their helpful advice on the real-time PCR analysis. We also thank Prof. I. Terashima for critical reading of the manuscript, and laboratory members for the kind advice, help and encouragement. This work was supported by KAKENHI (Grant-in-Aid for Scientific Research) on Priority Areas “Comparative Genomics” from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Supplementary material

10265_2010_342_MOESM1_ESM.xls (30 kb)
Table S1 (XLS 30 kb)


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Copyright information

© The Botanical Society of Japan and Springer 2010

Authors and Affiliations

  • Sachiko Funayama-Noguchi
    • 1
    Email author
  • Ko Noguchi
    • 1
  • Chie Yoshida
    • 1
    • 2
  • Masayoshi Kawaguchi
    • 2
  1. 1.Department of Biological Sciences, Graduate School of ScienceThe University of TokyoTokyoJapan
  2. 2.Division of Symbiotic SystemsNational Institute for Basic BiologyOkazakiJapan

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