Two CLE genes are induced by phosphate in roots of Lotus japonicus
- 403 Downloads
Genes of CLE (CLAVATA3/ESR-related) family encode peptide ligands that regulate plant development in response to external stimuli such as rhizobial infection and the nitrate application as well as various internal stimuli. To investigate whether LjCLE gene(s) may involve in plant response to inorganic phosphate (Pi), we analyzed Pi responses of 39 LjCLE genes in hydroponically grown Lotus japonicus plants (ecotype Miyakojima ‘MG-20’). Two LjCLE genes, LjCLE19 and 20, were up-regulated specifically and greatly in roots of L. japonicus by Pi addition to the hydroponic solution. When the external Pi level increased, expressions of LjCLE19 and 20 increased before the increase in the Pi content in plants. On the other hand, when the external Pi level decreased, the Pi content in plants decreased first, then expression levels of LjCLE19 and 20 decreased. Based on our results, we discuss the relationship between LjCLE19 and 20 and the tissue Pi levels in plants. This is the first report showing induction of specific CLE genes by phosphate.
KeywordsCLE genes Lotus japonicus Phosphate Root
We are grateful to Dr. M. Taira for the use of real-time PCR instrument, to Prof. T. Mimura for his advice on the measurement of Pi, to Dr. S. Yano for his advice on the measurement of root length and to Dr. S. Okamoto and Ms. E. Ohnishi for their helpful advice on the real-time PCR analysis. We also thank Prof. I. Terashima for critical reading of the manuscript, and laboratory members for the kind advice, help and encouragement. This work was supported by KAKENHI (Grant-in-Aid for Scientific Research) on Priority Areas “Comparative Genomics” from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
- Chapin III FS, Bieleski RL (1982) Mild phosphorus stress in barley and a related low-phosphorus-adapted barleygrass: phosphorus fractions and phosphate absorption in relation to growth. Physiol Plant 54:309–317. doi: 10.1111/j.1399-3054.1982.tb00264.x
- Colebatch G, Desbrosses G, Ott T, Krusell L, Montanari O, Kloska S, Kopka J, Udvardi MK (2004) Global changes in transcription orchestrate metabolic differentiation during symbiotic nitrogen fixation in Lotus japonicus. Plant J 39:487–512. doi: 10.1111/j.1365-313X.2004.02150.x CrossRefPubMedGoogle Scholar
- Hirakawa Y, Shinohara H, Kondo Y, Inoue A, Nakanomyo I, Ogawa M, Sawa S, Ohashi-Ito K, Matsubayashi Y, Fukuda H (2008) Non-cell-autonomous control of vascular stem cell fate by a CLE peptide/receptor system. Proc Natl Acad Sci USA 105:15208–15213. doi: 10.1073/pnas.0808444105 CrossRefPubMedGoogle Scholar
- Krusell L, Madsen LH, Sato S, Aubert G, Genua A, Szczyglowski K, Duc G, Kaneko T, Tabata S, de Bruijn F, Pajuelo E, Sandal N, Stougaard J (2002) Shoot control of root development and nodulation is mediated by a receptor-like kinase. Nature 420:422–426. doi: 10.1038/nature01207 CrossRefPubMedGoogle Scholar
- Morcuende R, Bari R, Gibon Y, Zheng W, Pant BD, Bläsing O, Usadel B, Czechowski T, Udvardi MK, Stitt M, Scheible W-R (2007) Genome-wide reprogramming of metabolism and regulatory networks of Arabidopsis in response to phosphorus. Plant Cell Environ 30:85–112. doi: 10.1111/j.1365-3040.2006.01608.x CrossRefPubMedGoogle Scholar
- Nakagawa T, Izumi T, Banba M, Umehara Y, Kouchi H, Izui K, Hata S (2003) Characterization and expression analysis of genes encoding phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxylase kinase of Lotus japonicus, a model legume. Mol Plant Microbe Interact 16:281–288. doi: 10.1094/MPMI.2003.16.4.281 CrossRefPubMedGoogle Scholar
- Plaxton WC, Carswell MC (1999) Metabolic aspects of the phosphate starvation response in plants. In: Lerner HR (ed) Plant responses to environmental stresses: from phytohormones to genome reorganization. Marcel Dekker, New York, pp 349–372Google Scholar
- Scheible W-R, Morcuende R, Czechowski T, Fritz C, Osuna D, Palacios-Rojas N, Schindelasch D, Thimm O, Udvardi MK, Stitt M (2004) Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen. Plant Physiol 136:2483–2499. doi: 10.1104/pp.104.047019 CrossRefPubMedGoogle Scholar
- Shane MW, Cramer MD, Funayama-Noguchi S, Cawthray GR, Millar AH, Day DA, Lambers H (2004) Developmental physiology of cluster-root carboxylate synthesis and exudation in harsh hakea. Expression of phosphoenolpyruvate carboxylase and the alternative oxidase. Plant Physiol 135:549–560. doi: 10.1104/pp.103.035659 CrossRefPubMedGoogle Scholar
- Sokal RR, Rohlf FJ (1995) Biometry, 3rd edn. WH Freeman, New YorkGoogle Scholar
- Strabala TJ, O’donnell PJ, Smit A-M, Ampomah-Dwamena C, Martin EJ, Netzler N, Nieuwenhuizen NJ, Quinn BD, Foote HCC, Hudson KR (2006) Gain-of function phenotypes of many CLAVATA3/ESR genes, including four new family members, correlate with tandem variations in the conserved CLAVATA3/ESR domain. Plant Physiol 140:1331–1344. doi: 10.1104/pp.105.075515 CrossRefPubMedGoogle Scholar