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Journal of Plant Research

, Volume 123, Issue 6, pp 789–799 | Cite as

Light-induced root hair formation in lettuce (Lactuca sativa L. cv. Grand Rapids) roots at low pH is brought by chlorogenic acid synthesis and sugar

  • Megumi Narukawa
  • Keiji Watanabe
  • Yasunori Inoue
Regular Paper

Abstract

Previously, we reported that chlorogenic acid (CGA) facilitated root hair formation at pH 4.0 in lettuce (Lactuca sativa L. cv. Grand Rapids). Light was essential for this process. In the present study, we determined relationships between CGA, light, and sugar during root hair formation in lettuce seedlings. The amount of CGA increased with white light in intact seedlings. Exogenously applied CGA restored root hair formation in dark-grown intact seedlings at pH 4.0. However, no root hair formation was induced in decapitated seedlings regardless of light exposure and CGA application. Application of sucrose or glucose induced both root hair formation and CGA synthesis in light-grown decapitated seedlings at pH 4.0. Blue light was the most effective for both root hair formation and CGA synthesis when supplied with sucrose to decapitated seedlings. Addition of sucrose and CGA together induced root hair formation at pH 4.0 in dark-grown decapitated seedlings. Results suggest that light induced CGA synthesis from sugar in the roots. Sugar was also required for root hair formation other than starting material of CGA synthesis. In addition, an unknown low pH-induced factor was essential for lettuce root hair formation.

Keywords

Blue light Chlorogenic acid Lettuce Low pH Root hair Sugar 

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Copyright information

© The Botanical Society of Japan and Springer 2010

Authors and Affiliations

  • Megumi Narukawa
    • 1
  • Keiji Watanabe
    • 2
  • Yasunori Inoue
    • 1
    • 3
  1. 1.Department of Applied Biological Science, Faculty of Science and TechnologyTokyo University of ScienceNodaJapan
  2. 2.Water and Soil Environment DivisionNational Institute for Environmental StudiesTsukubaJapan
  3. 3.Tissue Engineering Research Center, Research Institute of Biological ScienceTokyo University of ScienceNodaJapan

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