Development and characterization of polymorphic microsatellite markers in Linum usitatissimum
- 255 Downloads
Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.
KeywordsLinum usitatissimum Microsatellites marker Polymorphism
This work was funded by National Special Funds for Public Benefit Research of China (No. nyhyzx07-018-03) and Modern Agricultural Industry R&D System in China (No nycytx-19-E14).
- Li Q, Wan JM (2005) SSRHunter: development of a local searching software for SSR sites (in Chinese). Hereditas (Beijing) 27:808–810Google Scholar
- Raymond M, Rousset F (1995) Population genetics software for exact test and ecumenicism. J Heredity 86:248–249Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for genera users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana, Totowa, pp 365–386Google Scholar