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Journal of Plant Research

, 123:119 | Cite as

Development and characterization of polymorphic microsatellite markers in Linum usitatissimum

  • Xin Deng
  • Songhua Long
  • Dongfeng He
  • Xiang Li
  • Yufu Wang
  • Jia Liu
  • Xinbo Chen
Technical Note

Abstract

Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.

Keywords

Linum usitatissimum Microsatellites marker Polymorphism 

Notes

Acknowledgments

This work was funded by National Special Funds for Public Benefit Research of China (No. nyhyzx07-018-03) and Modern Agricultural Industry R&D System in China (No nycytx-19-E14).

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Copyright information

© The Botanical Society of Japan and Springer 2009

Authors and Affiliations

  • Xin Deng
    • 1
  • Songhua Long
    • 1
  • Dongfeng He
    • 2
  • Xiang Li
    • 2
  • Yufu Wang
    • 1
  • Jia Liu
    • 2
  • Xinbo Chen
    • 1
    • 2
  1. 1.Institute of Bast Fiber CropsChinese Academy of Agricultural SciencesChangshaChina
  2. 2.Hunan Provincial Key Laboratory of Crop Germplasm Innovation and UtilizationHunan Agricultural UniversityChangshaChina

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