Journal of Plant Research

, 122:623 | Cite as

Dramatic changes in leaf development of the native Capsicum chinense from the Seychelles at temperatures below 24°C

  • Sota Koeda
  • Munetaka Hosokawa
  • Byoung-Cheorl Kang
  • Susumu Yazawa
Regular Paper


When a pepper cultivar (Capsicum chinense cv. Seychelles-2, Sy-2) native to the Seychelles was grown in Japan, all seedlings showed seasonal developmental abnormalities such as development of abnormally shaped leaves. Other pepper cultivars grew well in all seasons while the growth of cv. Sy-2 was stunted. In this study, we first examined the effects of various changes in temperature and photoperiod on the cv. Sy-2 phenotype. The results showed that temperatures lower than 24°C led to the formation of abnormal leaves. Second, morphological and anatomical analyses of cotyledons and true leaves developed at 28 and 20°C were conducted. The narrower and thicker cotyledons developed at 20°C had fewer palisade cells in the leaf-length direction, and more cells in the leaf-thickness direction. True leaves developed at 20°C were irregularly shaped, thicker and had smaller leaf area. In addition, true leaves developed at 20°C had fewer palisade cells in the leaf-length and leaf-width directions and had more cells in the leaf-thickness direction. Furthermore, abnormal periclinal cell divisions in the mesophyll and/or epidermal cell layers were observed during leaf blade development at 20°C. These results suggest that the observed changes in cell proliferation and abnormal periclinal cell divisions were related, at least in part, to abnormal leaf development of cv. Sy-2 at temperatures below 24°C.


Capsicum chinense Cell division Leaf morphology Pepper Seychelles Temperature sensitivity 


  1. Baker BH, Miller JA (1963) Geology and geochronology of the Seychelles islands and structure of the floor of the Arabian sea. Nature 199:346–348CrossRefGoogle Scholar
  2. Deschamp PA, Cooke TJ (1983) Leaf dimorphism in aquatic angiosperms: significance of turgor pressure and cell expansion (Callitriche heterophylla). Science 219:505–507CrossRefPubMedGoogle Scholar
  3. Donnelly PM, Bonetta D, Tsukaya H, Dengler RE, Dengler NG (1999) Cell cycling and cell enlargement in developing leaves of Arabidopsis. Dev Biol 215:407–419CrossRefPubMedGoogle Scholar
  4. Dorland RE, Went FW (1947) Plant growth under controlled conditions. VIII. Growth and fruiting of the chilli pepper (Capsicum annuum). Am J Bot 34:393–401CrossRefGoogle Scholar
  5. Erwin JE, Heins RD, Karlsson MG (1989) Thermomorphogenesis in Lilium longiflorum. Am J Bot 76:47–52CrossRefGoogle Scholar
  6. Fleischmann K, Edwards PJ, Ramseier D, Kollmann J (2005) Stand structure, species diversity and regeneration of an endemic palm forest on the Seychelles. Afr J Ecol 43:291–301CrossRefGoogle Scholar
  7. Harrington JF, Kihara GM (1960) Chilling injury of germinating muskmelon and pepper seed. Proc Am Soc Hortic Sci 75:485–489Google Scholar
  8. Horiguchi G, Kim G-T, Tsukaya H (2005) The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana. Plant J 43:68–78CrossRefPubMedGoogle Scholar
  9. Ichimura K, Casais C, Peck SC, Shinozaki K, Shirasu K (2006) MEKK1 is required for MPK4 activation and regulates tissue-specific and temperature-dependent cell death in Arabidopsis. J Biol Chem 281:36969–36976CrossRefPubMedGoogle Scholar
  10. Johnson MP (1967) Temperature dependent leaf morphogenesis in Ranunculus flabellaris. Nature 214:1354–1355CrossRefGoogle Scholar
  11. Kane ME, Albert LS (1982) Environmental and growth regulator effects on heterophylly and growth of Proserpinaca intermedia (Haloragaceae). Aquat Bot 13:73–85CrossRefGoogle Scholar
  12. Knott JE, Deanon JR Jr (1967) Eggplant, tomato and pepper vegetable production in southeast asia, Los Banos, Laguna, Philippines. University of the Philippines, Los Banos PressGoogle Scholar
  13. Miller JA, Mudie JD (1961) Potassium–argon age determinations on granite from the island of Mahé in the Seychelles archipelago. Nature 192:1174–1175CrossRefGoogle Scholar
  14. Myster J, Moe R (1995) Effect of diurnal temperature alternations on plant morphology in some greenhouse crops—a mini review. Sci Hortic 62:205–215CrossRefGoogle Scholar
  15. Nonnecke LL (1989) Vegetable production. Van Nostrand Reinhold, New YorkGoogle Scholar
  16. Ozawa S, Yasutani I, Fukuda H, Komamine A, Sugiyama M (1998) Organogenic responses in tissue culture of srd mutants of Arabidopsis thaliana. Development 125:135–142PubMedGoogle Scholar
  17. Pickersgill B (1969) The archaeological record of chilli peppers (Capsicum spp.) and the sequence of plant domestication in Peru. Am Antiq 34:54–61CrossRefGoogle Scholar
  18. Pickett FB, Champagne MM, Meeks-Wagner DR (1996) Temperature-sensitive mutations that arrest Arabidopsis shoot development. Development 122:3799–3807PubMedGoogle Scholar
  19. Qi Y, Sun Y, Xu L, Xu Y, Huang H (2004) ERECTA is required for protection against heat-stress in the AS1/AS2 pathway to regulate adaxial–abaxial leaf polarity in Arabidopsis. Planta 219:270–276CrossRefPubMedGoogle Scholar
  20. Queitsch C, Sangstert TA, Lindquist S (2002) Hsp90 as a capacitor of phenotypic variation. Nature 417:618–624CrossRefPubMedGoogle Scholar
  21. Republic of Seychelles (2007) Seychelles in Figures, 2007 edn. (17 December 2008)
  22. Rutherford SL, Lindquist S (1998) Hsp90 as a capacitor for morphological evolution. Nature 396:336–342CrossRefPubMedGoogle Scholar
  23. Samuelsen AI, Rickson FR, Mok DWS, Mok MC (1997) A temperature-dependent morphological mutant of tobacco. Planta 201:303–310CrossRefPubMedGoogle Scholar
  24. Sangster TA, Salathia N, Lee HN, Watanabe E, Schellenberg K, Morneau K, Wang H, Undurraga S, Queitsch C, Lindquist S (2008) HSP90-buffered genetic variation is common in Arabidopsis thaliana. Proc Natl Acad Sci USA 105:2969–2974CrossRefPubMedGoogle Scholar
  25. Sato M, Tsutsumi M, Ohtsubo A, Nishii K, Kuwabara A, Nagata T (2008) Temperature-dependent changes of cell shape during heterophyllous leaf formation in Ludwigia arcuata (Onagraceae). Planta 228:27–36CrossRefPubMedGoogle Scholar
  26. Singh RJ (2007) Genetic resources, chromosome engineering, and crop improvement. Taylor and Francis, Boca RatonGoogle Scholar
  27. Swabey C (1970) The endemic flora of the Seychelle islands and its conservation. Biol Conserv 2:171–177CrossRefGoogle Scholar
  28. Thingnaes E, Torre S, Ernstsen A, Moe R (2003) Day and night temperature responses in Arabidopsis: effects on gibberellin and auxin content, cell size, morphology and flowering time. Ann Bot 92:601–612CrossRefPubMedGoogle Scholar
  29. Tsuge T, Tsukaya H, Uchimiya H (1996) Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh. Development 122:1589–1600PubMedGoogle Scholar
  30. Tsukaya H (2006) Mechanism of leaf-shape determination. Annu Rev Plant Biol 57:477–496CrossRefPubMedGoogle Scholar
  31. Tsukaya H, Inaba-Higano K, Komeda Y (1995) Phenotypic characterization and molecular mapping of an acaulis2 mutant of Arabidopsis thaliana with flower stalks of much reduced length. Plant Cell Physiol 36:239–244Google Scholar
  32. Waites R, Hudson A (1995) Phantastica: a gene required for dorsoventrality of leaves in Antirrhinum majus. Development 121:2143–2154Google Scholar
  33. Wallenstein A, Albert LS (1963) Plant morphology: its control in proserpinaca by photoperiod, temperature, and gibberellic acid. Science 140:998–1000CrossRefPubMedGoogle Scholar
  34. Wang CH (1990) Chilling injury of horticultural crops. Taylor and Francis, Boca RatonGoogle Scholar
  35. Went FW (1944) Plant growth under controlled conditions. II. Thermoperiodicity in growth and fruiting of the tomato. Am J Bot 31:135–150CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer 2009

Authors and Affiliations

  • Sota Koeda
    • 1
  • Munetaka Hosokawa
    • 1
  • Byoung-Cheorl Kang
    • 2
  • Susumu Yazawa
    • 1
  1. 1.Laboratory of Vegetable and Ornamental Horticulture, Department of Agronomy and Horticultural Science, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Department of Plant Science, College of Agriculture and Life SciencesSeoul National UniversitySeoulSouth Korea

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