Journal of Plant Research

, 122:235 | Cite as

Activation of abscisic acid biosynthesis in the leaves of Arabidopsis thaliana in response to water deficit

  • Keiichi Ikegami
  • Masanori Okamoto
  • Mitsunori Seo
  • Tomokazu Koshiba
Regular Paper


It is well known that endogenous abscisic acid (ABA) levels increase rapidly in response to drought stress and that this induces stomatal closure. In Arabidopsis thaliana, ABA levels increased rapidly in the leaves and roots when intact wild-type whole plants were exposed to drought stress. However, if the leaves and roots were separated and exposed to drought independently, the ABA level increased only in the leaves. These results suggest that, under our experimental conditions, ABA is synthesized mainly in the leaves in response to drought stress and that some of the ABA accumulated in the leaves is transported to the roots. Tracer experiments using isotope-labeled ABA indicate that the movement of ABA from leaves to roots is activated by water deficit in the roots. We also demonstrate that the endogenous ABA level in the leaves increased only when the leaves themselves were exposed to drought stress, suggesting that leaves play a major role in the production of ABA in response to acute water shortage.


Abiotic stress Abscisic acid (ABA) Arabidopsisthaliana Biosynthesis Stomatal closure Transport 



We thank Dr. Tadao Asami (University of Tokyo) for kindly providing the 13C-labeled ABA, and Dr. Shinjiro Yamaguchi (RIKEN Plant Science Center) for critical reading of this manuscript.


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Copyright information

© The Botanical Society of Japan and Springer 2008

Authors and Affiliations

  • Keiichi Ikegami
    • 1
  • Masanori Okamoto
    • 1
    • 2
  • Mitsunori Seo
    • 2
    • 3
  • Tomokazu Koshiba
    • 1
  1. 1.Department of Biological SciencesTokyo Metropolitan UniversityTokyoJapan
  2. 2.Dormancy and Adaptation Research UnitRIKEN Plant Science CenterKanagawaJapan
  3. 3.Laboratoire de Biologie des SemencesUMR204 AgroParisTech, Institut Jean-Pierre BourginVersailles CedexFrance

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