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Journal of Plant Research

, Volume 121, Issue 5, pp 493–498 | Cite as

Transient increase in the transcript levels of γ-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls

  • Kouichi SogaEmail author
  • Toshihisa Kotake
  • Kazuyuki Wakabayashi
  • Seiichiro Kamisaka
  • Takayuki Hoson
Short Communication

Abstract

By hypergravity treatment, the percentage of cells with transverse microtubules was decreased, while that with longitudinal microtubules was increased in azuki bean (Vigna angularis) epicotyls. The expression of genes encoding γ-tubulin complex (VaTUG and VaGCP3) was increased transiently in response to changes in the gravitational conditions. Lanthanum and gadolinium ions, potential blockers of mechanosensitive calcium ion-permeable channels (mechanoreceptors), nullified reorientation of microtubules as well as up-regulation of expression of VaTUG and VaGCP3 by hypergravity. These results suggest that mechanoreceptors may perceive the gravity signal, which leads to a transient increase in the transcript levels of γ-tubulin complex genes and reorientation of cortical microtubules.

Keywords

Azuki bean (Vigna angularis Ohwi et Ohashi) Epicotyl Gravity Microtubule γ-Tubulin complex 

Notes

Acknowledgments

We thank Professor H. Numata of Osaka City University for his advice on statistical analysis. The present study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, a Grant for Ground-based Research for Space Utilization from the Japan Space Forum, and by Sasakawa Scientific Research Grant from the Japan Science Society.

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Copyright information

© The Botanical Society of Japan and Springer 2008

Authors and Affiliations

  • Kouichi Soga
    • 1
    Email author
  • Toshihisa Kotake
    • 2
  • Kazuyuki Wakabayashi
    • 1
  • Seiichiro Kamisaka
    • 3
  • Takayuki Hoson
    • 1
  1. 1.Department of Biology and Geosciences, Graduate School of ScienceOsaka City UniversityOsakaJapan
  2. 2.Division of Life Science, Graduate School of Science and EngineeringSaitama UniversitySaitamaJapan
  3. 3.Graduate School of Science and EngineeringUniversity of ToyamaToyamaJapan

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